Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

polymer vectors for gene therapy has been studied as an alternative to viral vectors. In particular, double hydrophilic block copolymers (DHBCs) has shown potential in this domain, but until now research mainly focuses on non-degradable copolymer, which may be restrictions for further development.

To overcome these limitations, we synthesized DHBC (PEG43-b-PCL12 (COOH) 6.5) composed of poly (ethylene glycol) (PEG) non-ionic and bioeliminable block and carboxylic poly acid-functionalized degradable (ε-caprolactone) (PCL) block. DHBC potential as a genuine vector for small interfering ribonucleic acid (siRNA) to formulate tripartite polyionic complex (PIC) micelles with poly (lysine) (PLL) is evaluated.

We first study the impact of the cost ratio (R) on the size and zeta potential of micelles produced. With the cost ratio of R = 1, the formulations with optimized physico-chemical properties demonstrated the ability to complex 75% of the siRNA. We demonstrate the stability of the micelles at pH 7.4 and at pH 5 disturbance, which allows the pH-triggered release of siRNA and proven character of the pH-responsive micelles tripartite stimuli.

In addition, the PIC micelles tripartite non-cytotoxic proven below 40 ug / mL. Potential siRNA vector is then evaluated in vitro: it was found that the tripartite PIC micelles are allowed siRNA internalization into 3 times higher than the PLL polyplexes in murine mesenchymal stem cells, and is able to transfect human breast cancer cells. Overall, these data sets pre-validate the use of degradable DHBC as non-viral vectors for the encapsulation and controlled release of siRNA, which is why it may be a sound alternative for non-degradable and / or cytotoxic polycationic vector.

Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery
Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

Circular role Ribonucleic Acid in the Treatment of Traumatic Brain and Spinal Cord Injury

The causal and pathogenetic factors associated with traumatic brain injury (TBI) and spinal cord injury (SCI) complex. This complexity is a contributory factor in the outcome of a minimal increase of individuals injured. Several studies have demonstrated the potential of stem cells to facilitate neuronal growth and improve results in a variety of neurological conditions, including TBI and SCI. However, the efficiency of the delivery of stem cells at the injured site is open to question.

Circular ribonucleic acid (RNA circular) have recently become the focus of attention because of the different functions and features in vivo. Exosomes have a close relationship with a circular RNA and may have a significant impact on the circular RNA expression.

Here, we review the circular RNA specific functions, exosomes, and stem cells in the central nervous system conditions, mainly TBI and SCI, and trying to show the relationship between them. Our analysis shows a circular RNA’s role in the brain / spinal cord injury for a multifaceted, as they can modulate several signaling mechanisms while also acting as a sponge for microRNAs and bind to their sites. In addition, the circular RNA can activate some biological activity, molecular and cellular in the wake of injury to the brain bone / spine. Thus, both non-pharmacological interventions and pharmacological regulation centered on a circular RNA can promise to TBI and SCI.

Purified Influenza A Neuraminidase (H1N1-NA) protein control for western blot

H1N1NA11-C 100 ul
EUR 286

Core Panel Multi-Tumor control slides

TS900 Set of 5
EUR 218

Core Panel Multi-Tumor control slides

TS900-25 Set of 25
EUR 485

Breast Panel Multi-Tumor control slides

TS901 Set of 5
EUR 218

Breast Panel Multi-Tumor control slides

TS901-25 Set of 25
EUR 485

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 103

BSA Control for Age-BSA

2221-BSA
EUR 175

BSA Control for AGE-BSA

35R-AA007 10 mg
EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Control for DOG1, 3 cases (1.5mm)

DOG1061 1
EUR 85
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for Ckit, 6 samples (1.5mm)

CKIT061 1
EUR 85
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Control for HER2, 4 cases (1.5mm)

HRC081 1
EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

SLLK, Control Peptide for TSP1 Inhibitor

HY-P0301 1mg
EUR 133

Control for ER, 4 cases (1.5mm)

ERC081 1
EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for PR, 3 cases (1.5mm)

PRC061 1
EUR 85
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-100 100 Slides
EUR 706

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-25 25 Slides
EUR 232

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-5 5 Slides
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Thiostatin Rat protein, control for western

TSTN11-C 100 ul
EUR 286

Control/Blocking peptide for Human WNT5a

WNT511-P 100 ug
EUR 164

Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA

20021-1 100 ug
EUR 225

Elk IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-1 100 ug
EUR 225

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-2 100 ug
EUR 225

Bison IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20025-1 100 ug
EUR 225

Raccoon IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20026-1 100 ug
EUR 225

Skunk IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20027-1 100 ug
EUR 225

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange 1 prep
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Mouse IgG (-ve control for flow cytometry)

20008-1-100 100 tests
EUR 103

Rabbit IgG (-ve control for flow cytometry)

20009-1-100 100 tests
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Human Apolipoprotein B protein control for WB

APOB21-C 100 ul
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Rec. Human CD21 protein control for WB

CD21-C 100 ul
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ADA11-C 100 ul
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Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
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Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
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Haptoglobin Human Plasma protein control for WB

HGLB18-C 100 ul
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Human Hemopexin purified protein control for Western

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Hemopexin Mouse, purified protein control for Western

HPEX16-C 100 ul
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SLLK, Control Peptide for TSP1 Inhibitor(TFA)

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FLGN17-C 100 ul
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NCM802M 7 ml
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Control/Blocking peptide for Ephrin-B2 antibody

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OVA11-C 100 ul
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Control Sections for FD NeuroSilver™ Kit

PCS101 each
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Positive Control for Anti-Human CYP1B1 Antibody

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Purified, Bovine S100 protein control for WB

S1001-C 100 ul
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Purified, Human S100 protein control for WB

S1002-C 100 ul
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Control/Blocking peptide for Mouse Vimentin (Vim)

VIM11-C 100 ug
EUR 164

Control/Blocking peptide for Human WNT-1

WNT111-P 100 ug
EUR 457

Control siRNA Vector (pGB-control)

9500C-20
EUR 338

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H1N1-02-A 100 ul
EUR 482

Mouse Anti-Cardiolipin Ig's -ve control for ELISA

5502 1 ml
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Human Apolipoprotein C-I protein control for WB

APOC11-C 100 ul
EUR 286

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APOC22-C 100 ul
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CECA15-C 100 ul
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Positive Control for Anti-Human Cytochrome b5 Antibody

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ACTB15-C 100 ul
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Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
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ALBC15-C 100 ul
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Monkey (Rhesus) Serum Albumin protein control for WB

ALBMK21-C 100 ul
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Monkey (Rhesus) Serum Albumin protein control for WB

ALBMK22-C 100 ul
EUR 286

Chicken Ovomucoid (trypsin inhibitor) protein control for Western

COVM13-C 100 ul
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Control/Blocking peptide for Mouse Histone deacetylase 1

HDAC111-P 100 ug
EUR 164

Purified Human Neutrophil Elastase protein control for WB

ESTE11-C 100 ul
EUR 286

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P1ACON 100 uL
EUR 125

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Purified Nipah virus Nucleoprotein control for Western Blotting

NIV21-C 100 ul
EUR 225

Myoglobin, Human Cardiac, purified protein control for western

MYG17-C 100 ul
EUR 286

Human Prothrombin (Factor II) protein control for Western

PRTN11-C 100 ul
EUR 286

Mouse Prothrombin (Factor II) protein control for Western

PRTN13-C 100 ul
EUR 286

Control sections for FD NeuroApop™ Kit (paraffin)

PCS102-1 each
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PCS102-2 each
EUR 67.12
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Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 286

Human Salivary Amylase protein positive control for WB

SAMY16-C 100 ul
EUR 286

Purified Human Survivin protein for WB +ve control

SURV11-C 100 ul
EUR 286

Purified, recombinant Human TACE control protein for WB

TACE11-C 100 ul
EUR 286

Control sections for FD NeuroApop™ Kit (frozen)

PCS102- each
EUR 73

Quality Control

abx098966-1vial 1 vial
EUR 300
  • Shipped within 5-7 working days.

pMD18- Control

PVT10563 2 ug
EUR 266

pMD19- Control

PVT10564 2 ug
EUR 266

NATtrol Influenza A H1N1 Positive Control (6 X 0.5 mL)

NATFLUAH1N1-6MC 6 X 0.5 mL
EUR 232
  • What is the product classification?
  • NATtrol Influenza A H1N1 Positive Control is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.

Influenza A virus (H1N1) matrix protein 2 Control/blocking peptide

M2E16-P 100 ug
EUR 164


Disease coronavirus (COVID-19), which is caused by a coronavirus 2 acute respiratory syndrome (SARS-CoV-2), has been declared a pandemic. We here report the four COVID-19 cases with long-term positive viral ribonucleic acid (RNA) for about 61 days. Although treatment with human recombinant interferon, plasma recovered from COVID-19 patients, Arbidol, etc., the results of nucleic acid is still positive for SARS-CoV-2. After treatment with ritonavir danoprevir (DNVr, 100/100 mg, once daily), four patients showed 2-3 consecutive negative SARS-CoV-2 RNA and thus out of the hospital.

Kathleen

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