Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

polymer vectors for gene therapy has been studied as an alternative to viral vectors. In particular, double hydrophilic block copolymers (DHBCs) has shown potential in this domain, but until now research mainly focuses on non-degradable copolymer, which may be restrictions for further development.

To overcome these limitations, we synthesized DHBC (PEG43-b-PCL12 (COOH) 6.5) composed of poly (ethylene glycol) (PEG) non-ionic and bioeliminable block and carboxylic poly acid-functionalized degradable (ε-caprolactone) (PCL) block. DHBC potential as a genuine vector for small interfering ribonucleic acid (siRNA) to formulate tripartite polyionic complex (PIC) micelles with poly (lysine) (PLL) is evaluated.

We first study the impact of the cost ratio (R) on the size and zeta potential of micelles produced. With the cost ratio of R = 1, the formulations with optimized physico-chemical properties demonstrated the ability to complex 75% of the siRNA. We demonstrate the stability of the micelles at pH 7.4 and at pH 5 disturbance, which allows the pH-triggered release of siRNA and proven character of the pH-responsive micelles tripartite stimuli.

In addition, the PIC micelles tripartite non-cytotoxic proven below 40 ug / mL. Potential siRNA vector is then evaluated in vitro: it was found that the tripartite PIC micelles are allowed siRNA internalization into 3 times higher than the PLL polyplexes in murine mesenchymal stem cells, and is able to transfect human breast cancer cells. Overall, these data sets pre-validate the use of degradable DHBC as non-viral vectors for the encapsulation and controlled release of siRNA, which is why it may be a sound alternative for non-degradable and / or cytotoxic polycationic vector.

Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery
Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

Circular role Ribonucleic Acid in the Treatment of Traumatic Brain and Spinal Cord Injury

The causal and pathogenetic factors associated with traumatic brain injury (TBI) and spinal cord injury (SCI) complex. This complexity is a contributory factor in the outcome of a minimal increase of individuals injured. Several studies have demonstrated the potential of stem cells to facilitate neuronal growth and improve results in a variety of neurological conditions, including TBI and SCI. However, the efficiency of the delivery of stem cells at the injured site is open to question.

Circular ribonucleic acid (RNA circular) have recently become the focus of attention because of the different functions and features in vivo. Exosomes have a close relationship with a circular RNA and may have a significant impact on the circular RNA expression.

Here, we review the circular RNA specific functions, exosomes, and stem cells in the central nervous system conditions, mainly TBI and SCI, and trying to show the relationship between them. Our analysis shows a circular RNA’s role in the brain / spinal cord injury for a multifaceted, as they can modulate several signaling mechanisms while also acting as a sponge for microRNAs and bind to their sites. In addition, the circular RNA can activate some biological activity, molecular and cellular in the wake of injury to the brain bone / spine. Thus, both non-pharmacological interventions and pharmacological regulation centered on a circular RNA can promise to TBI and SCI.

Recombinant Purified Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) protein control for Western

H1N1-01-C 100 ul
EUR 343.2

Influenza A virus (H1N1) matrix protein 2 Control/blocking peptide

M2E16-P 100 ug
EUR 196.8

Recombinant Purified Hemagglutinin Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) protein control for Western

H11N2-01-C 100 ul
EUR 343.2

Influenza A virus (H5N1) matrix protein 2 Control/blocking peptide

M2E15-P 100 ug
EUR 196.8

Influenza A virus (H9N2) matrix protein 2 Control/blocking peptide

M2E17-P 100 ug
EUR 196.8

Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A/chicken/India/NIV33487/2006) (17-531aa) protein control for Western

H5N11-C 100 ul
EUR 343.2

NATtrol Influenza Negative Control (6 X 0.5 mL)

NATCXVA9-6MC 6 X 0.5 mL
EUR 220

NATtrol Influenza/RSV Negative Control (6 x 0.5mL)

NATCXVA9-6C 6 x 0.5mL
EUR 220

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

NATCXVA9-6C-IVD 6 X 0.5 mL
EUR 240

NATtrol Influenza A/B Positive Control (6 x 0.5mL)

NATFLUAB-6C 6 x 0.5mL
EUR 211

NATtrol Influenza/RSV Positive Control (6 x 0.5 mL)

NATFLURSV-6C 6 x 0.5 mL
EUR 221

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

MDZ045 6 X 0.5 mL
EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza/RSV Positive Control (6 x 0.25mL)

NATFLURSV-6L 6 x 0.25mL
EUR 272

NATtrol Influenza A/B Positive Control (6 X 0.5 mL)

NATFLUA/B-6MC 6 X 0.5 mL
EUR 227

NATtrol Influenza A/B  Positive Control (6 X 0.5 mL)

NATFLUAB-6C-IVD 6 X 0.5 mL
EUR 231

NATtrol Influenza A/B Positive Control (6 X 0.5 mL)

MDZ046 6 X 0.5 mL
EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

Influenza A virus (H5N1) matrix protein 2 hybrid sequence Control/blocking peptide

M2E11-P 100 ug
EUR 196.8

Influenza A Virus IgA Control Serum

C1231A 3 mL
EUR 105

Influenza A Virus IgG Control Serum

C1231G 3 mL
EUR 105

Influenza A Virus IgM Control Serum

C1231M 3 mL
EUR 105

Influenza B Virus IgA Control Serum

C1232A 3 mL
EUR 105

Influenza B Virus IgG Control Serum

C1232G 3 mL
EUR 105

Influenza B Virus IgM Control Serum

C1232M 3 mL
EUR 105

NATtrol Influenza B, External Run Control, Low (6 X 1 mL)

NATFLUB-ERCL 6 X 1 mL
EUR 360

NATtrol Influenza A H1, External Run Control, Low (6 X 1 mL)

NATFLUAH1-ERCL 6 X 1 mL
EUR 360

Influenza Rapid Test Control Pack (3 X 1.5mL)

KZMC033 3 X 1.5mL
EUR 59

NATtrol Influenza B, External Run Control, Medium (6 X 1 mL)

NATFLUB-ERCM 6 X 1 mL
EUR 438

NATtrol Influenza A H1, External Run Control, Medium (6 X 1 mL)

NATFLUAH1-ERCM 6 X 1 mL
EUR 438

Influenza A virus HA Control/blocking peptide

AB-23091-P 100ug
EUR 196.8

Influenza-A Hemagglutinin H1N1 (Influenza-A H1N1) Antibody

abx137717-100tests 100 tests
EUR 225

Influenza-A H1N1

ant-214 10µg
EUR 60
Description: Mouse Anti Influenza-A Hemagglutinin H1N1

Influenza-A H1N1 PAT1G7AT

ant-754 5µg
EUR 60
Description: Mouse Anti Influenza-A Hemagglutinin H1N1 Clone PAT1G7AT

Influenza-A H1N1 Antibody

20-abx137503
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ug
  • 15 ug
  • 5 ug

Influenza-A H1N1 Antibody

20-abx137508
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  • 10 ug
  • 1 mg
  • 50 ug

Human anti-H. Influenzae B (Hib-PRP) IgG negative control serum

980-101-HNC 1 ml
EUR 196.8

Human anti-H. Influenzae B (Hib-PRP) IgG positive control serum

980-101-HPC 1 ml
EUR 270

Recombinant H. Influenzae protein D (Non-typeable, NT-PHiD) controls for western blot

PHID11-C 100 ul
EUR 343.2

Human anti-H. Influenzae B protein D (Hib-PDG) IgG negative control serum

980-200-01N 1 ml
EUR 196.8

Human anti-H. Influenzae B protein D (Hib-PDG) IgG positive control serum

980-200-02P 1 ml
EUR 270

H1N1 Influenza-A Virus Taiwan/1/86

rAP-5393 Inquiry Ask for price

H1N1 Influenza-A Virus Beijing/262/95

rAP-5388 Inquiry Ask for price

Core Panel Multi-Tumor control slides, set of 5

TS900 Set of 5
EUR 145
Description: Core Panel Multi-Tumor control slides, set of 5

Core Panel Multi-Tumor control slides, set of 25

TS900-25 Set of 25
EUR 425
Description: Core Panel Multi-Tumor control slides, set of 25

H1N1 Influenza-A Virus Solomon Islands/03/06

rAP-5408 Inquiry Ask for price

Breast Panel Multi-Tumor control slides, set of 5

TS901 Set of 5
EUR 145
Description: Breast Panel Multi-Tumor control slides, set of 5

Breast Panel Multi-Tumor control slides, set of 25

TS901-25 Set of 25
EUR 395
Description: Breast Panel Multi-Tumor control slides, set of 25

NATtrol Influenza Verification Panel (18 x 0.5mL)

NATFVP(XP)-C 18 x 0.5mL
EUR 722

Casein, for Molecular Biology

MB279-100G 1 unit
EUR 110.77
Description: Casein, for Molecular Biology

Casein, for Molecular Biology

MB279-500G 1 unit
EUR 387.65
Description: Casein, for Molecular Biology

NATtrol Influenza Verification Panel (7 x 1.0 mL)

NATFVP-JAN 7 x 1.0 mL
EUR 569.18
Description: Please contact Gentaur in order to receive the datasheet of the product.

H1N1 Influenza-A Virus New Caledonia/20/99 IVR 116

rAP-5389 Inquiry Ask for price

Control Animal Genomic DNA Panel, Set of any 5 species

G-005 5X0.025mg
EUR 720

Control Animal Genomic DNA Panel, Set of any 10 species

G-010 10x0.025mg
EUR 1193

NATtrol Influenza/RSV Verification Panel (21 x 0.5mL)

NATFRVP-C 21 x 0.5mL
EUR 862

NATIVE INFLUENZA A H1N1 ANTIGEN

GWB-B63944 1 ml Ask for price

Influenza A H1N1

GWB-B42E6F 1 ml Ask for price

Influenza А H1N1

Oneq-H442-OneqH442100R Oneq-H442-100R
EUR 1028

Influenza А H1N1

Oneq-H442-OneqH442150R Oneq-H442-150R
EUR 1438

Influenza А H1N1

Oneq-H442-OneqH44250R Oneq-H442-50R
EUR 728

Influenza А H1N1

PCR-H442-PCRH44248R PCR-H442-48R
EUR 485

Influenza А H1N1

PCR-H442-PCRH44296R PCR-H442-96R
EUR 685

genesig Easy kit for Influenza A H7N9

Z-Path-H7N9-EASY 50 tests
EUR 410
Description: H7N9

H1N1 Influenza-A Virus New Caledonia/20/99 Recombinant

rAP-5387 Inquiry Ask for price

genesig Easy kit for Equine influenza (H3N8_2019)

Z-Path-H3N8_2019-EASY 50 tests
EUR 410
Description: H3N8_2019

H1N1 Influenza-A Virus Solomon Islands/03/06 Recombinant

rAP-5417 Inquiry Ask for price

Influenza A Virus Subtype H1N1 (H1N1) Antibody

abx411425-1ml 1 ml
EUR 610.8

Influenza A Virus Subtype H1N1 (H1N1) Antibody

abx415737-01ml 0.1 ml
EUR 961.2

Influenza A Virus Subtype H1N1 (H1N1) Antibody

abx411425-100g 100 µg Ask for price

Influenza A Virus Subtype H1N1 (H1N1) Antibody

abx411425-250g 250 µg Ask for price

Influenza A Virus Subtype H1N1 (H1N1) Antibody

abx411425-25g 25 µg
EUR 537.5

D-(+)-Glucose, anhydrous ?For Molecular

MB037-100G 1 unit
EUR 17.24
Description: D-(+)-Glucose, anhydrous ?For Molecular

D-(+)-Glucose, anhydrous ?For Molecular

MB037-500G 1 unit
EUR 83.6
Description: D-(+)-Glucose, anhydrous ?For Molecular

Urea, suitable for molecular biology

GE1210-1 1
EUR 58

Urea, suitable for molecular biology

GE1210-1KG 1 kg
EUR 106.8

Urea, suitable for molecular biology

GE1210-500 500
EUR 33.1

Urea, suitable for molecular biology

GE1210-500G 500 g
EUR 76.8

Influenza A (H1N1) -FITC

GWB-10EA90 1 ml Ask for price

Influenza A H1N1 -FITC

GWB-13C149 1 ml Ask for price

D(+)-Lactose monohydrate ?For Molecular

MB062-500G 1 unit Ask for price
Description: D(+)-Lactose monohydrate ?For Molecular

D(+)-Lactose monohydrate ?For Molecular

MB062-5KG 1 unit Ask for price
Description: D(+)-Lactose monohydrate ?For Molecular

Influenza A virus Subtype H1N1 (A/H1N1) Antibody

abx137508-01mg 0.1 mg
EUR 225

Influenza Virus A H1N1

GWB-FBB9AE 1 mg Ask for price

Influenza A Virus Subtype H1N1 (H1N1) Antibody (HRP)

abx411426-1ml 1 ml
EUR 760.8

Influenza A Strain (H1N1)

GWB-9856E4 1 ml Ask for price

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve

GS8780-2500 2500
EUR 249.8

Influenza A (H1N1) Antigen

11-553 1 mL
EUR 645
Description: Influenza A, Influenza A (H1N1)

Influenza A H1N1 Antigen

abx670268-1ml 1 ml
EUR 627.6

Influenza A (H1N1) Protein

abx061588-1ml 1 ml
EUR 794.4

Influenza A (H1N1) Protein

abx061588-100g 100 µg Ask for price

Influenza A (H1N1) Protein

abx061588-10g 10 µg
EUR 862.5

Influenza A (H1N1) Protein

abx061588-50g 50 µg Ask for price

Influenza A H1N1 Antigen

abx670268-100g 100 µg
EUR 437.5

Control for Tyrosinase, 4 cases (1.5mm)HumanskinSkinIHC control

TYROSIN081 1.5mm
EUR 50

Guanidine hydrochloride ?For Molecular B

MB014-100G 1 unit
EUR 72.26
Description: Guanidine hydrochloride ?For Molecular B

Guanidine hydrochloride ?For Molecular B

MB014-500G 1 unit
EUR 325.05
Description: Guanidine hydrochloride ?For Molecular B

Guanidine thiocyanate?For Molecular Biol

MB015-1KG 1 unit
EUR 118.81
Description: Guanidine thiocyanate?For Molecular Biol

Guanidine thiocyanate?For Molecular Biol

MB015-500G 1 unit
EUR 79.2
Description: Guanidine thiocyanate?For Molecular Biol

BSA Control for Age-BSA

2221-BSA each
EUR 210

BSA Control for AGE-BSA

35R-AA007 10 mg Ask for price
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 123.6

Influenza A H1N1 antibody

10R-7791 50 ug
EUR 678
Description: Mouse monoclonal Influenza A H1N1 antibody

Influenza A antibody (H1N1)

20-IG23 1 mg
EUR 359
Description: Goat polyclonal Influenza A antibody (H1N1)

Influenza A antibody (H1N1)

20C-CR1233GP 1 ml
EUR 360
Description: Goat polyclonal Influenza A antibody (H1N1)

Influenza A H1N1 Antibody

GWB-4FE1DD 1 ml Ask for price

Influenza A H1N1 Antibody

abx023045-100ug 100 ug
EUR 1011.6

Influenza A H1N1 Antibody

abx023046-100ug 100 ug
EUR 961.2

Influenza A H1N1 Antibody

GWB-A880F0 1 mg Ask for price

Influenza A H1N1 Antibody

GWB-DC4916 0.1 ml Ask for price

Influenza A H1N1 Antibody

GWB-87427B 1 mg Ask for price

Influenza А H1N1 PCR kit

PCR-H442-48R 50T
EUR 987.6

Influenza А H1N1 PCR kit

PCR-H442-96R 100T
EUR 1335.6

2-Mercaptoethanol ?For Molecular Biology

MB041-100ML 1 unit
EUR 9.02
Description: 2-Mercaptoethanol ?For Molecular Biology

2-Mercaptoethanol ?For Molecular Biology

MB041-500ML 1 unit
EUR 26.47
Description: 2-Mercaptoethanol ?For Molecular Biology

Control for Mammaglobin, 4 cases (1.5mm)HumanBreastBreastIHC control

MAMA-G081 1.5mm
EUR 50

Influenza A H1N1 NA (Active)

E40VAG027 20ug
EUR 495
Description: LFA, EIA,CLIA,WB

NZCYM HiVeg Medium (For Molecular Biolog

GV017-500G 1 unit
EUR 63.29
Description: NZCYM HiVeg Medium (For Molecular Biolog

genesig Easy kit for Equine/Canine influenza (H3N8 & H7N7)

Z-Path-H3N8-H7N7-EASY 50 tests
EUR 410
Description: H3N8&H7N7

Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve

GS3406-2500 2500
EUR 116.2

Myc-DDK-tagged ORF clone of viral ORF for HA1 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, YP_163

VC102476 10 µg Ask for price

Myc-DDK-tagged ORF clone of viral ORF for HA2 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expression, YP_163

VC102477 10 µg Ask for price

Influenza А H1N1 RT PCR kit

RTq-H442-100R 100T
EUR 1573.2

Influenza А H1N1 RT PCR kit

RTq-H442-150R 150T
EUR 2144.4

Influenza А H1N1 RT PCR kit

RTq-H442-50R 50T
EUR 1155.6

Influenza Rapid Test Verification Panel (20 X 0.5 mL)

KZMC021 20 X 0.5 mL
EUR 286

Negative Control for Mouse IgG1

MC-950 NCG01 Ask for price

Negative Control for Rabbit IgG

TA353974 100 µl Ask for price

Negative Control for Rabbit IgG

PC-124 Polyclonal Ask for price

Control for GCDFP-15, 4 cases (1.5mm)HumanBreastBreastIHC control

GCDFP081 1.5mm
EUR 50

Influenza A antibody (H1N1) (HRP)

60-I30 1 ml
EUR 420
Description: Goat polyclonal Influenza A antibody (H1N1) (HRP) conjugated

Influenza A H1N1 HA1 antibody

10R-7995 100 ug
EUR 338
Description: Mouse monoclonal Influenza A H1N1 HA1 antibody

Influenza A H1N1 Antibody (HRP)

GWB-C7F495 1 ml Ask for price

Control Peptide for SNAPtideÒ #520

525 200nmoles
EUR 600

Control for MSH2, 4 cases (1.5mm)HumanDigestive systemColonIHC control

MSH2081 1.5mm
EUR 50

Control for Olig2, 4 cases (1.5mm)HumanNervous systemBrainIHC control

OLIG2081 1.5mm
EUR 50

Influenza A antibody (H1N1) (FITC)

60C-CR1233GF 1 ml
EUR 385
Description: Goat polyclonal Influenza A antibody (H1N1) (FITC) conjugated

Influenza A antibody (H1N1) (FITC)

60-I32 1 ml
EUR 385
Description: Goat polyclonal Influenza A antibody (H1N1) (FITC) conjugated

Influenza Virus A H1N1 Beijing

GWB-5848DD 1 mg Ask for price

Influenza A H1N1 Antibody (FITC)

GWB-A3EF8E 1 ml Ask for price

Influenza A Virus (subtype H1N1)

RA19071 100 ul
EUR 568.8

Control for ER, 4 cases (1.5mm)

ERC081 1
EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for PR, 3 cases (1.5mm)

PRC061 1
EUR 102
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

Influenza-A H5N1

ant-073 5µg
EUR 60
Description: Mouse Anti-Human H5N1HA1

Influenza-A H3N2

ant-080 5µg
EUR 60
Description: Mouse Anti-Human H3N2HA1

Influenza-A H3N2

ant-215 10µg
EUR 60
Description: Mouse Anti Influenza-A Hemagglutinin H3N2

Influenza-A H5N1

ant-216 10µg
EUR 60
Description: Mouse Anti Influenza-A Hemagglutinin H5N1

Influenza-A H5N1

ant-535 5µg
EUR 60
Description: Polyclonal Rabbit Anti Influenza-A Hemagglutinin H5N1

Scrambled Control for Pri Peptide

069-78 200 μg
EUR 160.92

Influenza A (A/Beijing/262/95)(H1N1)

GWB-9F69BF 1 mg Ask for price

Influenza A H1N1 protein (Taiwan)

30R-AI038 1 mg
EUR 1320
Description: Purified native Influenza A H1N1 protein (Taiwan)

Influenza A (H1N1) Antigen Liquid

IFLUAH1N1ALR1ML each
EUR 771
Description: Influenza A (H1N1) Antigen Liquid

H3N2 Influenza-A Virus Panama/2007/99

rAP-5392 Inquiry Ask for price

Myc-DDK-tagged ORF clone of viral ORF for neuraminidase [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expressi

VC102468 10 µg Ask for price

Myc-DDK-tagged ORF clone of viral ORF for haemagglutinin [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell express

VC102475 10 µg Ask for price

QPCR Kit RNA H1N1 influenza - EACH

MOL6930 EACH
EUR 1712.62

Influenza A H1N1 protein (Beijing)

30R-AI039 1 mg
EUR 1320
Description: Purified native Influenza A H1N1 protein (Beijing)

Control for DOG1, 3 cases (1.5mm)

DOG1061 1
EUR 102
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for HER2, 4 cases (1.5mm)

HRC081 1
EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Myc-DDK-tagged ORF clone of viral ORF for matrix protein 1 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expre

VC102473 10 µg Ask for price

Myc-DDK-tagged ORF clone of viral ORF for matrix protein 2 [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell expre

VC102474 10 µg Ask for price

Myc-DDK-tagged ORF clone of viral ORF for PB1-F2 protein [Influenza A virus (A/Puerto Rico/8/1934(H1N1))], codon optimized for human cell express

VC102480 10 µg Ask for price

NATtrol Respiratory Panel 2 (RP2) Controls (Ea)

NATRPC2-BIO Ea
EUR 550.46
Description: Please contact Gentaur in order to receive the datasheet of the product.


Disease coronavirus (COVID-19), which is caused by a coronavirus 2 acute respiratory syndrome (SARS-CoV-2), has been declared a pandemic. We here report the four COVID-19 cases with long-term positive viral ribonucleic acid (RNA) for about 61 days. Although treatment with human recombinant interferon, plasma recovered from COVID-19 patients, Arbidol, etc., the results of nucleic acid is still positive for SARS-CoV-2. After treatment with ritonavir danoprevir (DNVr, 100/100 mg, once daily), four patients showed 2-3 consecutive negative SARS-CoV-2 RNA and thus out of the hospital.

Kathleen

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