Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

polymer vectors for gene therapy has been studied as an alternative to viral vectors. In particular, double hydrophilic block copolymers (DHBCs) has shown potential in this domain, but until now research mainly focuses on non-degradable copolymer, which may be restrictions for further development.

To overcome these limitations, we synthesized DHBC (PEG43-b-PCL12 (COOH) 6.5) composed of poly (ethylene glycol) (PEG) non-ionic and bioeliminable block and carboxylic poly acid-functionalized degradable (ε-caprolactone) (PCL) block. DHBC potential as a genuine vector for small interfering ribonucleic acid (siRNA) to formulate tripartite polyionic complex (PIC) micelles with poly (lysine) (PLL) is evaluated.

We first study the impact of the cost ratio (R) on the size and zeta potential of micelles produced. With the cost ratio of R = 1, the formulations with optimized physico-chemical properties demonstrated the ability to complex 75% of the siRNA. We demonstrate the stability of the micelles at pH 7.4 and at pH 5 disturbance, which allows the pH-triggered release of siRNA and proven character of the pH-responsive micelles tripartite stimuli.

In addition, the PIC micelles tripartite non-cytotoxic proven below 40 ug / mL. Potential siRNA vector is then evaluated in vitro: it was found that the tripartite PIC micelles are allowed siRNA internalization into 3 times higher than the PLL polyplexes in murine mesenchymal stem cells, and is able to transfect human breast cancer cells. Overall, these data sets pre-validate the use of degradable DHBC as non-viral vectors for the encapsulation and controlled release of siRNA, which is why it may be a sound alternative for non-degradable and / or cytotoxic polycationic vector.

Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery
Degradable double hydrophilic block copolymers and tripartite polyionic complex micelles thereof for small interfering ribonucleic acids (siRNA) delivery

Circular role Ribonucleic Acid in the Treatment of Traumatic Brain and Spinal Cord Injury

The causal and pathogenetic factors associated with traumatic brain injury (TBI) and spinal cord injury (SCI) complex. This complexity is a contributory factor in the outcome of a minimal increase of individuals injured. Several studies have demonstrated the potential of stem cells to facilitate neuronal growth and improve results in a variety of neurological conditions, including TBI and SCI. However, the efficiency of the delivery of stem cells at the injured site is open to question.

Circular ribonucleic acid (RNA circular) have recently become the focus of attention because of the different functions and features in vivo. Exosomes have a close relationship with a circular RNA and may have a significant impact on the circular RNA expression.

Here, we review the circular RNA specific functions, exosomes, and stem cells in the central nervous system conditions, mainly TBI and SCI, and trying to show the relationship between them. Our analysis shows a circular RNA’s role in the brain / spinal cord injury for a multifaceted, as they can modulate several signaling mechanisms while also acting as a sponge for microRNAs and bind to their sites. In addition, the circular RNA can activate some biological activity, molecular and cellular in the wake of injury to the brain bone / spine. Thus, both non-pharmacological interventions and pharmacological regulation centered on a circular RNA can promise to TBI and SCI.

Influenza A virus (H1N1) matrix protein 2 Control/blocking peptide

M2E16-P 100 ug
EUR 196.8

Recombinant Purified Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) protein control for Western

H1N1-01-C 100 ul
EUR 343.2

Influenza A virus (H5N1) matrix protein 2 Control/blocking peptide

M2E15-P 100 ug
EUR 196.8

Influenza A virus (H9N2) matrix protein 2 Control/blocking peptide

M2E17-P 100 ug
EUR 196.8

NATtrol Influenza Negative Control (6 X 0.5 mL)

NATCXVA9-6MC 6 X 0.5 mL
EUR 220

Recombinant Purified Hemagglutinin Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) protein control for Western

H11N2-01-C 100 ul
EUR 343.2

NATtrol Influenza/RSV Negative Control (6 x 0.5mL)

NATCXVA9-6C 6 x 0.5mL
EUR 220

NATtrol Influenza/RSV Positive Control (6 x 0.25mL)

NATFLURSV-6L 6 x 0.25mL
EUR 272

NATtrol Influenza A/B Positive Control (6 x 0.5mL)

NATFLUAB-6C 6 x 0.5mL
EUR 211

NATtrol Influenza/RSV Positive Control (6 x 0.5 mL)

NATFLURSV-6C 6 x 0.5 mL
EUR 221

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

NATCXVA9-6C-IVD 6 X 0.5 mL
EUR 240

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

MDZ045 6 X 0.5 mL
EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza A/B Positive Control (6 X 0.5 mL)

NATFLUA/B-6MC 6 X 0.5 mL
EUR 227

NATtrol Influenza A/B  Positive Control (6 X 0.5 mL)

NATFLUAB-6C-IVD 6 X 0.5 mL
EUR 231

NATtrol Influenza A/B Positive Control (6 X 0.5 mL)

MDZ046 6 X 0.5 mL
EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

Influenza Rapid Test Control Pack (3 X 1.5mL)

KZMC033 3 X 1.5mL
EUR 59

NATtrol Influenza B, External Run Control, Low (6 X 1 mL)

NATFLUB-ERCL 6 X 1 mL
EUR 360

NATtrol Influenza A H1, External Run Control, Low (6 X 1 mL)

NATFLUAH1-ERCL 6 X 1 mL
EUR 360

Influenza A virus HA Control/blocking peptide

AB-23091-P 100ug
EUR 196.8

Influenza A virus (H5N1) matrix protein 2 hybrid sequence Control/blocking peptide

M2E11-P 100 ug
EUR 196.8

NATtrol Influenza B, External Run Control, Medium (6 X 1 mL)

NATFLUB-ERCM 6 X 1 mL
EUR 438

Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A/chicken/India/NIV33487/2006) (17-531aa) protein control for Western

H5N11-C 100 ul
EUR 343.2

NATtrol Influenza A H1, External Run Control, Medium (6 X 1 mL)

NATFLUAH1-ERCM 6 X 1 mL
EUR 438

Human anti-H. Influenzae B (Hib-PRP) IgG negative control serum

980-101-HNC 1 ml
EUR 196.8

Human anti-H. Influenzae B (Hib-PRP) IgG positive control serum

980-101-HPC 1 ml
EUR 270

Human anti-H. Influenzae B protein D (Hib-PDG) IgG negative control serum

980-200-01N 1 ml
EUR 196.8

Human anti-H. Influenzae B protein D (Hib-PDG) IgG positive control serum

980-200-02P 1 ml
EUR 270

Core Panel Multi-Tumor control slides, set of 5

TS900 Set of 5
EUR 145
Description: Core Panel Multi-Tumor control slides, set of 5

Core Panel Multi-Tumor control slides, set of 25

TS900-25 Set of 25
EUR 425
Description: Core Panel Multi-Tumor control slides, set of 25

Breast Panel Multi-Tumor control slides, set of 5

TS901 Set of 5
EUR 145
Description: Breast Panel Multi-Tumor control slides, set of 5

Breast Panel Multi-Tumor control slides, set of 25

TS901-25 Set of 25
EUR 395
Description: Breast Panel Multi-Tumor control slides, set of 25

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 123.6

Recombinant H. Influenzae protein D (Non-typeable, NT-PHiD) controls for western blot

PHID11-C 100 ul
EUR 343.2

BSA Control for Age-BSA

2221-BSA each
EUR 210

BSA Control for AGE-BSA

35R-AA007 10 mg
EUR 268.8
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Flow Control Fitting for Flypad

FLY1254 EACH
EUR 101.84

Control for ER, 4 cases (1.5mm)

ERC081 1
EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for PR, 3 cases (1.5mm)

PRC061 1
EUR 102
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

Flow Control Fitting for Blowgun

FLY1252 EACH
EUR 80.95

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-2 100 ug
EUR 270

Control for DOG1, 3 cases (1.5mm)

DOG1061 1
EUR 102
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for HER2, 4 cases (1.5mm)

HRC081 1
EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Control for Ckit, 6 samples (1.5mm)

CKIT061 1
EUR 102
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

Influenza-A H1N1 Antibody

20-abx137503
  • EUR 1128.00
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 15 ug
  • 5 ug

Influenza-A H1N1 Antibody

20-abx137508
  • EUR 276.00
  • EUR 2148.00
  • EUR 393.60
  • 10 ug
  • 1 mg
  • 50 ug

SLLK, Control Peptide for TSP1 Inhibitor

HY-P0301 1mg
EUR 159.6

Control/Blocking peptide for Human WNT5a

WNT511-P 100 ug
EUR 196.8

Rec. Human CD21 protein control for WB

CD21-C 100 ul
EUR 343.2

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-100 100 Slides
EUR 847.2

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-25 25 Slides
EUR 278.4

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-5 5 Slides
EUR 117.6

Thiostatin Rat protein, control for western

TSTN11-C 100 ul
EUR 343.2

Mouse IgG (-ve control for flow cytometry)

20008-1-100 100 tests
EUR 123.6

Rabbit IgG (-ve control for flow cytometry)

20009-1-100 100 tests
EUR 123.6

NATtrol Respiratory Panel 2 (RP2) Controls (Ea)

NATRPC2-BIO Ea
EUR 550.46
Description: Please contact Gentaur in order to receive the datasheet of the product.

Control Sections for FD NeuroSilver™ Kit

PCS101 each
EUR 69.6

Purified, Human S100 protein control for WB

S1002-C 100 ul
EUR 343.2

Control/Blocking peptide for Human WNT-1

WNT111-P 100 ug
EUR 548.4

Negative Control Antibody for Mouse Monoclonals

V8785-100UG 100ug
EUR 349.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Negative Control Antibody for Mouse Monoclonals

V8785-20UG 20ug
EUR 153.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Negative Control Antibody for Mouse Monoclonals

V8785SAF-100UG 100ug
EUR 349.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

SLLK, Control Peptide for TSP1 Inhibitor(TFA)

HY-P0301A 5mg
EUR 456

Purified, Bovine S100 protein control for WB

S1001-C 100 ul
EUR 343.2

Positive Control for Anti-Rat MEH Antibody

MEHCON 100 uL
EUR 150

Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 343.2

Human Apolipoprotein B protein control for WB

APOB21-C 100 ul
EUR 343.2

Positive Control for Anti-Rat CYP2C Antibody

P2CCON 100 uL
EUR 150

Positive Control for Anti-Rat CYP2D Antibody

P2DCON 100 uL
EUR 150

Positive Control for Anti-Rat CYP2B1 Antibody

P2B1PTCON 100 uL
EUR 150

Positive Control for Anti-Rat CYP3A2 Antibody

P3A2PTCON 100 uL
EUR 150

Haptoglobin Human Plasma protein control for WB

HGLB18-C 100 ul
EUR 343.2

Control/Blocking peptide for Mouse Vimentin (Vim)

VIM11-C 100 ug
EUR 196.8

NATtrol Respiratory Panel 2.1 (RP2.1) Controls (12 x 0.3mL)

NATRPC2.1-BIO 12 x 0.3mL
EUR 529

Mouse IgG Isotype control (purified) validated for

NG903 7 ml
EUR 195
Description: Mouse IgG Isotype control (purified) validated for IHC; Ready-To-Use

Mouse IgG Isotype control (purified) validated for

NG903C 1 ml
EUR 275
Description: Mouse IgG Isotype control (purified) validated for IHC; Concentrate; Minimum 1:50 dilution

Mouse IgM Isotype control (purified) validated for

NG906 7 ml
EUR 265
Description: Mouse IgM Isotype control (purified) validated for IHC; Ready-To-Use

Mouse IgM Isotype control (purified) validated for

NG906C 1 ml
EUR 295
Description: Mouse IgM Isotype control (purified) validated for IHC; Concentrate

Positive Control for Anti-Human CYP3A Antibody

P3ACON 100 uL
EUR 150

Eppendorf Centrifuge 5430R Knob Control Refrigerated- IVD Only

E5428000465 EACH
EUR 8115.6

Control/Blocking peptide for Ephrin-B2 antibody

NIVE2B-C 100 ug
EUR 196.8

Positive Control for Anti-Human CYP1B1 Antibody

PH1B1CON 100 uL
EUR 150

Positive Control for Anti-Human CYP2E1 Antibody

P2E1PTCON 100 uL
EUR 150

Positive Control for Anti-Human CYP1A2 Antibody

P1A2CON 100 uL
EUR 150

Chicken Egg Ovalbumin protein control for Western

OVA11-C 100 ul
EUR 343.2

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 343.2

Positive control tissue section for each individua

Control-slides- Set of 5
EUR 119
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Positive control tissue section for each individua

Control-Slides-for-each-antibody Set of 25
EUR 355
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Control sections for FD NeuroApop™ Kit (frozen)

PCS102- each
EUR 87.6

Control sections for FD NeuroApop™ Kit (frozen)

PCS102-2 each
EUR 80.54
Description: Best quality products

Human Native Retinol binding protein (RBP) control for Western

RBP41-C 100 ul
EUR 343.2

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 343.2

Purified Flagellin (salmonella) protein control for WB

FLGN17-C 100 ul
EUR 343.2

Human Hemopexin purified protein control for Western

HPEX11-C 100 ul
EUR 343.2

Hemopexin Mouse, purified protein control for Western

HPEX16-C 100 ul
EUR 343.2

Q-VE-OPh (Negative Control for Q-VD-OPh)

GW8990-1 1
EUR 228.1

Lectin binding native protein (positive control for SNA lectin)

LBP16-N-1 1 ml
EUR 270

Human Apolipoprotein C-I protein control for WB

APOC11-C 100 ul
EUR 343.2

Control sections for FD NeuroApop™ Kit (paraffin)

PCS102-1 each
EUR 80.44
Description: Best quality products

Purified Human Survivin protein for WB +ve control

SURV11-C 100 ul
EUR 343.2

Lectin binding native protein (negative control for all lectins)

LNP15-N-1 1 ml
EUR 270

Human Apolipoprotein C-II protein control for WB

APOC22-C 100 ul
EUR 343.2

Monkey (Rhesus) Serum Albumin protein control for WB

ALBMK21-C 100 ul
EUR 343.2

Monkey (Rhesus) Serum Albumin protein control for WB

ALBMK22-C 100 ul
EUR 343.2

Positive Control for Anti-Rat CHP2B1/2 Antibody

P2B12PTCON 100 uL
EUR 150

Bovine Adenosine deaminase protein control for Western

ADA11-C 100 ul
EUR 343.2

Positive Control for anti-GST p-forms: rat P

GSTCON3 100 uL
EUR 150

Human Apolipoprotein C-III protein control for WB

APOC32-C 100 ul
EUR 343.2

Purified human Catalase (CATL) protein control for WB

CATL11-C 100 ul
EUR 343.2

Positive Control for Anti-Rat CYP1A1/1A2 Antibody

P1ACON 100 uL
EUR 150

Purified bovine Lactoferrin protein control for Western

LTF13-C 100 ul
EUR 343.2

Rat neurofascin (Nfasc)-control Control/blocking peptide

AB-23249-CP 100ug
EUR 196.8

Chicken Egg white proteins total control for Western

CEGW12-C 100 ul
EUR 343.2

Rabbit Beta Actin (muscle) protein control for Western

ACTB15-C 100 ul
EUR 388.8

Human plasma Apolipoprotein E protein control for WB

APOE13-C 100 ul
EUR 343.2

Human Plasma Apolipoprotein J protein control for WB

APOJ13-C 100 ul
EUR 343.2

Chicken Egg yellow proteins total control for Western

CEYP12-C 100 ul
EUR 343.2

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 343.2

Human Salivary Amylase protein positive control for WB

SAMY16-C 100 ul
EUR 343.2

Purified, recombinant Human TACE control protein for WB

TACE11-C 100 ul
EUR 343.2

pMD18- Control

PVT10563 2 ug
EUR 319.2

pMD19- Control

PVT10564 2 ug
EUR 319.2

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 343.2

Human Prothrombin (Factor II) protein control for Western

PRTN11-C 100 ul
EUR 343.2

Mouse Prothrombin (Factor II) protein control for Western

PRTN13-C 100 ul
EUR 343.2

Mouse Angiopoietin 3 (Ang-3) protein control for WB

ANG31-C 100 ul
EUR 343.2

Human Angiopoietin 4 (Ang-4) protein control for WB

ANG41-C 100 ul
EUR 343.2

Purified Rat C-Reactive Protein (CRP) control for WB

CRP16-C 100 ul
EUR 343.2

Purified Dog C-Reactive Protein (CRP) control for WB

CRP18-C 100 ul
EUR 343.2

Lectin binding native protein (positive control for ConA, WGA lectins)

LBP15-N-1 1 ml
EUR 270

Positive Control for Anti-Human Cytochrome b5 Antibody

Cb5CON 100 uL
EUR 150

Recombinant (CHO) human DNAse I Protein control for WB

DNASE12-C 100 ul
EUR 343.2

Positive Control for anti-GST m-forms: rat M1 (Yb)

GSTCON2 100 uL
EUR 150

Quality Control

abx098966-1vial 1 vial
EUR 360

Purified Human Neutrophil Elastase protein control for WB

ESTE11-C 100 ul
EUR 343.2

Control/Blocking peptide for Mouse Histone deacetylase 1

HDAC111-P 100 ug
EUR 196.8

Purified Human C-Reactive Protein (CRP) control for WB

CRP12-C 100 ul
EUR 343.2

Troponin-T, Human Cardiac, pure protein control for WB

TNTC55-C 100 ul
EUR 343.2

Positive Control for anti-GST m-forms: rat M2 (Yb2)

GSTCON21 100 uL
EUR 150

Rat IgG-PE conjugate (isotype control) (Isotype control)

20005-PE 25 tests
EUR 242.4

Rat IgG-HRP conjugate (isotype control) (Isotype control)

20005-HP 100 ug
EUR 196.8

Camelpox virus H3L/p35 protein control for western blot

CPOX11-C 100 ul
EUR 343.2

Alpha 1-Antitrypsin (A1AT), Human protein control for WB

A1AT11-C 100 ul
EUR 343.2

Zika Virus prM Protein (African) control for Western blot

ZPRM11-C 100 ul
EUR 343.2

Mouse Serum albumin control (level 1 ~ 1ug/ml) for ELISA

6300-C1 1 ml
EUR 169.2

Rat IgG-FITC conjugate (isotype control) (Isotype control)

20005-F 100 ug
EUR 196.8

Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
EUR 343.2

H 104 cover for RET basic; RET control and RCT basic

STI2902 EACH
EUR 28.8

PIPET CONTROLLER CONTROL BOARD

357484 1/pk
EUR 104.4
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

Chicken Egg proteins total (whole egg) control for Western

CEGT11-C 100 ul
EUR 343.2

Alpha 2-Macroglobulin (A2M), Human protein control for WB

A2MG11-C 100 ul
EUR 343.2

Maltose binding protein (MBP) (fusion tag) control for ELISA

MBP15-R-100 100 ug
EUR 416.4

Maltose binding protein (MBP) (fusion tag) control for ELISA

MBP15-R-1000 1 mg
EUR 927.6

Rat IgG-Biotin conjugate (isotype control) (Isotype control)

20005-B 100 ug
EUR 196.8

Myoglobin, Human Cardiac, purified protein control for western

MYG17-C 100 ul
EUR 343.2

Apolipoprotein A-Il, Human Plasma protein control for WB

APOA21-C 100 ul
EUR 343.2

Human recombinant purified BMP-1 protein control for WB

BMP12-C 100 ul
EUR 343.2

Human Liver Cathepsin B protein control for Western blot

CTHB11-C 100 ul
EUR 343.2

Human Liver Cathepsin D protein control for Western blot

CTHD11-C 100 ul
EUR 343.2

Human Liver Cathepsin G protein control for Western blot

CTHG11-C 100 ul
EUR 343.2

Human Liver Cathepsin H protein control for Western blot

CTHH11-C 100 ul
EUR 343.2

Human Liver Cathepsin L protein control for Western blot

CTHL11-C 100 ul
EUR 343.2


Disease coronavirus (COVID-19), which is caused by a coronavirus 2 acute respiratory syndrome (SARS-CoV-2), has been declared a pandemic. We here report the four COVID-19 cases with long-term positive viral ribonucleic acid (RNA) for about 61 days. Although treatment with human recombinant interferon, plasma recovered from COVID-19 patients, Arbidol, etc., the results of nucleic acid is still positive for SARS-CoV-2. After treatment with ritonavir danoprevir (DNVr, 100/100 mg, once daily), four patients showed 2-3 consecutive negative SARS-CoV-2 RNA and thus out of the hospital.

Kathleen

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