“Zooming in” on Glioblastoma: Understanding Tumor Heterogeneity and its Clinical Implications in the Era of Single-Cell Ribonucleic Acid Sequencing

“Zooming in” on Glioblastoma: Understanding Tumor Heterogeneity and its Clinical Implications in the Era of Single-Cell Ribonucleic Acid Sequencing

Glioblastoma (GBM) is the most common primary brain malignancy in adults and one of the most aggressive of all cancers in humans. It is very repetitive and treatment-resistant, largely because the infiltrative nature and inter- and intratumoral heterogeneity. This heterogeneity requires different landscape genomics and cell types within and between the tumor and the tumor microenvironment (TME). In the GBM, is the driving resistance heterogeneity of treatment, recurrence, and prognosis is poor, represent a major obstacle to personalized medicine.

Over the past decade, the sequencing technology has facilitated a deeper understanding of GBM heterogeneity by “zooming in” more progressive on tumor genomics and transcriptomics. initial efforts to use mass ribonucleic acid (RNA) sequencing, which examined the combined gene expression of tumor specimens as a whole. While innovative at the time, this bulk RNAseq mask important contribution of sub-populations of different tumor’s gene expression as a whole.

This work evolved into mass use RNA sequencing on tumor subsections anatomically and spatially distinct, previously unappreciated complexity exhibited genome of GBM. The revolutionary next step forward has been the emergence of a single cell RNA sequencing (scRNAseq), which examined the gene expression at the single cell level. scRNAseq has enabled us to understand in detail the heterogeneity GBM unprecedented.

We review studies of seminal in the development of our understanding of GBM heterogeneity, with a focus on scRNAseq and insight that have been provided in the understanding of GBM tumor mass, peritumoral space, and TME. We highlight the preclinical and clinical implications of this work and consider the potential impact of neuro-oncology, and to improve patient outcomes through personalized medicine.

"Zooming in" on Glioblastoma: Understanding Tumor Heterogeneity and its Clinical Implications in the Era of Single-Cell Ribonucleic Acid Sequencing
“Zooming in” on Glioblastoma: Understanding Tumor Heterogeneity and its Clinical Implications in the Era of Single-Cell Ribonucleic Acid Sequencing

[Knockdown of Fez family of zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) inhibits invasion and migration of esophageal squamous cell carcinoma cells by blocking the JAK2 / STAT3 pathway]

Objective To investigate the relationship between the long chain of non-coding RNA Fez family of zinc protein finger 1 antisense ribonucleic acid 1 (FEZF1-AS1) and invasion, migration and Janus kinase 2 / signal transducer and activator of transcription 3 (JAK2 / STAT3) signaling pathway in carcinoma squamous cell (ESCC) cells of the esophagus. Methods The expression ‘of FEZF1-AS1 in cancer tissues and cell lines ESCC ESCC was detected by real-time quantitative PCR.

After knocking out FEZF1-AS1 of KYSE150 and KYSE510 cells with expression vectors lentiviral built, changes in cell cycle were detected by flow cytometry, the ability of cell proliferation by colony test formations, the ability of cell invasion by TranswellTM invasion assay, the ability of cell migration by TranswellTM migration and early test, and the level of phosphorylated proteins JAK2 and STAT3 (p-STAT3) by Western blotting.

NATtrol Influenza A/B  Positive Control (6 X 0.5 mL)

NATFLUAB-6C-IVD 6 X 0.5 mL
EUR 231

NATtrol Influenza A/B Positive Control (6 X 0.5 mL)

MDZ046 6 X 0.5 mL
EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

Influenza A virus HA Control/blocking peptide

AB-23091-P 100ug
EUR 196.8

NATtrol Influenza A H1N1 Positive Control (6 X 0.5 mL)

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EUR 227

Recombinant Purified Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) protein control for Western

H1N1-01-C 100 ul
EUR 343.2

NATtrol Influenza Negative Control (6 X 0.5 mL)

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EUR 220

Influenza B Virus IgA Control Serum

C1232A 3 mL
EUR 105

Influenza B Virus IgG Control Serum

C1232G 3 mL
EUR 105

Influenza B Virus IgM Control Serum

C1232M 3 mL
EUR 105

NATtrol Influenza/RSV Negative Control (6 x 0.5mL)

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EUR 220

NATtrol Influenza A H1N1, External Run Control, Low (6 X 1 mL)

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EUR 360

NATtrol Influenza/RSV Positive Control (6 x 0.25mL)

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EUR 272

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

NATCXVA9-6C-IVD 6 X 0.5 mL
EUR 240

NATtrol Influenza/RSV Positive Control (6 x 0.5 mL)

NATFLURSV-6C 6 x 0.5 mL
EUR 221

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)

MDZ045 6 X 0.5 mL
EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza A H1N1, External Run Control, Medium (6 X 1 mL)

NATFLUAH1N1-ERCM 6 X 1 mL
EUR 438

Recombinant Purified Hemagglutinin Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) protein control for Western

H11N2-01-C 100 ul
EUR 343.2

Influenza Rapid Test Control Pack (3 X 1.5mL)

KZMC033 3 X 1.5mL
EUR 59

NATtrol Influenza B, External Run Control, Low (6 X 1 mL)

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NATtrol Influenza B, External Run Control, Medium (6 X 1 mL)

NATFLUB-ERCM 6 X 1 mL
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Influenza A virus (H5N1) matrix protein 2 Control/blocking peptide

M2E15-P 100 ug
EUR 196.8

Influenza A virus (H1N1) matrix protein 2 Control/blocking peptide

M2E16-P 100 ug
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Influenza A virus (H9N2) matrix protein 2 Control/blocking peptide

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Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A/chicken/India/NIV33487/2006) (17-531aa) protein control for Western

H5N11-C 100 ul
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Influenza A virus (H5N1) matrix protein 2 hybrid sequence Control/blocking peptide

M2E11-P 100 ug
EUR 196.8

NATtrol Influenza Verification Panel (18 x 0.5mL)

NATFVP(XP)-C 18 x 0.5mL
EUR 722

NATtrol Influenza Verification Panel (7 x 1.0 mL)

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Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza/RSV Verification Panel (21 x 0.5mL)

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Human anti-H. Influenzae B (Hib-PRP) IgG negative control serum

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Human anti-H. Influenzae B (Hib-PRP) IgG positive control serum

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Mouse antibody for Influenza A virus

1343 100 ug
EUR 431.93
Description: This is Mouse monoclonal FITC conjugated antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus

1333 100 ug
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Description: This is Mouse monoclonal FITC conjugated antibody against Influenza A virus NP for WB, ELISA.

Goat polyclonal antibody for Influenza A

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EUR 386.26
Description: This is HRP conjugated goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Goat polyclonal antibody for Influenza A

1301 1 ml
EUR 327.53
Description: This is goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

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1303 1 ml
EUR 347.1
Description: This is FITC conjugated goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Goat polyclonal antibody for Influenza A

1304 1 ml
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Description: This is HRP conjugated goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

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1307 1 ml
EUR 347.1
Description: This is Biotin conjugated goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Goat polyclonal antibody for Influenza A

1311 1 ml
EUR 327.53
Description: This is goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A

1313 1 ml
EUR 347.1
Description: This is FITC conjugated goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A

1314 1 ml
EUR 373.2
Description: This is HRP conjugated goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

Goat polyclonal antibody for Influenza A

1317 1 ml
EUR 347.1
Description: This is Biotin conjugated goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A

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EUR 327.53
Description: This is goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

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EUR 347.1
Description: This is FITC conjugated goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

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Description: This is Biotin conjugated goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

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Description: H7N9

Mouse antibody for Influenza A virus NP

1341 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP

1361 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP

1371 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP

1381 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus H5

1385 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus H5 for WB, ELISA.

Mouse antibody for Influenza A virus NP

1331 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

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Influenza A H1 Antibody

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Influenza A H1 Antibody

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EUR 1161.6

Influenza A H1 Antibody

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EUR 1161.6

Influenza A H1 Antibody

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Influenza A H1 Antibody

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Influenza A H1 Antibody

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Description: Core Panel Multi-Tumor control slides, set of 5

Core Panel Multi-Tumor control slides, set of 25

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EUR 425
Description: Core Panel Multi-Tumor control slides, set of 25

Breast Panel Multi-Tumor control slides, set of 5

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EUR 395
Description: Breast Panel Multi-Tumor control slides, set of 25

Casein, for Molecular Biology

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EUR 110.77
Description: Casein, for Molecular Biology

Casein, for Molecular Biology

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Influenza A HA H1 antibody

10-7839 500 ug
EUR 242
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Influenza A HA H1 antibody

10-7840 500 ug
EUR 240
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Control Animal Genomic DNA Panel, Set of any 5 species

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EUR 1193

Influenza A H1 (Swine) Peptide

abx060603-005mg 0.05 mg
EUR 393.6

Influenza A H1 (Swine) Peptide

abx060603-100g 100 µg Ask for price

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abx060603-200g 200 µg Ask for price

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abx060603-50g 50 µg
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Influenza A H1 Antigen Antibody

GWB-563399 0.1 mg Ask for price

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Influenza A virus H1 PCR kit

PCR-H648-48R 50T
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Influenza A virus H1 PCR kit

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Influenza A Hemagglutinin H1 Antibody

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D-(+)-Glucose, anhydrous ?For Molecular

MB037-100G 1 unit
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D-(+)-Glucose, anhydrous ?For Molecular

MB037-500G 1 unit
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Description: D-(+)-Glucose, anhydrous ?For Molecular

Urea, suitable for molecular biology

GE1210-1 1
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Urea, suitable for molecular biology

GE1210-1KG 1 kg
EUR 106.8

Urea, suitable for molecular biology

GE1210-500 500
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Urea, suitable for molecular biology

GE1210-500G 500 g
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D(+)-Lactose monohydrate ?For Molecular

MB062-500G 1 unit Ask for price
Description: D(+)-Lactose monohydrate ?For Molecular

D(+)-Lactose monohydrate ?For Molecular

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Description: D(+)-Lactose monohydrate ?For Molecular

Control for Napsin A, 4 cases (1.5mm)HumanMulti-systemMulti-organsIHC control

NAPSINA081 1.5mm
EUR 50

H. influenza DNA primase - for 100 assays

DNAG-100HI 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM H. influenzae primase-helicase complex

NATtrol Influenza A H1 Stock (Quantitative) (1 mL)

NATFLUAH1-STQ 1 mL
EUR 857

Goat polyclonal antibody for Influenza B

1101 1 ml
EUR 327.53
Description: This is goat polyclonal antibody against Influenza B virions for WB, ELISA.

Goat polyclonal antibody for Influenza B

1103 1 ml
EUR 347.1
Description: This is FITC conjugated goat polyclonal antibody against Influenza B virions for WB, ELISA.

Goat polyclonal antibody for Influenza B

1324 1 ml
EUR 373.2
Description: This is HRP conjugated goat polyclonal antibody against Influenza B virions for WB, ELISA.

Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve

GS8780-2500 2500
EUR 249.8

H. influenza DNA helicase - for 100 assays

DNAB-100HI 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM H influenzae DNA helicase.

Control for Tyrosinase, 4 cases (1.5mm)HumanskinSkinIHC control

TYROSIN081 1.5mm
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Guanidine hydrochloride ?For Molecular B

MB014-100G 1 unit
EUR 72.26
Description: Guanidine hydrochloride ?For Molecular B

Guanidine hydrochloride ?For Molecular B

MB014-500G 1 unit
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Guanidine thiocyanate?For Molecular Biol

MB015-1KG 1 unit
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Description: Guanidine thiocyanate?For Molecular Biol

Guanidine thiocyanate?For Molecular Biol

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Influenza A Virus H1 Antigen Antibody

abx415739-01mg 0.1 mg
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Influenza A Virus H1 Antigen Antibody

abx415739-200l 200 µl
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BSA Control for Age-BSA

2221-BSA each
EUR 210

BSA Control for AGE-BSA

35R-AA007 10 mg Ask for price
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 123.6

Control for Chromogranin A, 4 cases (1.5mm)HumanNervous systemMulti-organsIHC control

CHRA081 1.5mm
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Influenza A virus H1 RT PCR kit

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Influenza A virus H1 RT PCR kit

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Influenza A virus H1 RT PCR kit

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2-Mercaptoethanol ?For Molecular Biology

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EUR 9.02
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EUR 26.47
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Mouse antibody for Influenza B virus NP

1161 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza B virus NP for WB, ELISA.

Mouse antibody for Influenza B virus NP

1181 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza B virus NP for WB, ELISA.

Mouse antibody for Influenza B virus NP

1171 100 ug
EUR 386.26
Description: This is purified Mouse monoclonal antibody against Influenza B virus NP for WB, ELISA.

genesig Real-time PCR detection kit for Influenza A H7N9

Z-Path-H7N9 150 tests
EUR 808
Description: H7N9

Control for Mammaglobin, 4 cases (1.5mm)HumanBreastBreastIHC control

MAMA-G081 1.5mm
EUR 50

ELISA kit for Human Influenza A virus (FluA) antibody (IgA)

EK0232 96 tests
EUR 854.4
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Influenza A virus (FluA) antibody (IgA) in samples from serum, plasma, tissue homogenates and other biological fluids.

genesig Easy kit for Human Influenza A Virus Subtype H3

Z-Path-H3-EASY 50 tests
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Description: H3

genesig Easy kit for Avian Influenza A Virus Subtype H7

Z-Path-H7-EASY 50 tests
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Description: H7

NZCYM HiVeg Medium (For Molecular Biolog

GV017-500G 1 unit
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Description: NZCYM HiVeg Medium (For Molecular Biolog

Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve

GS3406-2500 2500
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Negative Control for Mouse IgG1

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Negative Control for Rabbit IgG

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Negative Control for Rabbit IgG

PC-124 Polyclonal Ask for price

Control for GCDFP-15, 4 cases (1.5mm)HumanBreastBreastIHC control

GCDFP081 1.5mm
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Results FEZF1-AS1 are highly expressed in cancer tissues ESCC and FEZF1-AS1 expression in cells KYSE510 KYSE150 and higher than that in normal esophageal squamous epithelial cells. Upon knockdown FEZF1-AS1 expression, cell cycle and proliferation of cancer cells ESCC did not change obviously, but cell invasion and migration was significantly inhibited, and the protein levels decreased JAK2 and p-STAT3 protein increases. Conclusion FEZF1-AS1 knockdown blocks JAK2 / STAT3 pathway and inhibits cell invasion and migration of ESCC.

Kathleen

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