Glioblastoma (GBM) is the most common primary brain malignancy in adults and one of the most aggressive of all cancers in humans. It is very repetitive and treatment-resistant, largely because the infiltrative nature and inter- and intratumoral heterogeneity. This heterogeneity requires different landscape genomics and cell types within and between the tumor and the tumor microenvironment (TME). In the GBM, is the driving resistance heterogeneity of treatment, recurrence, and prognosis is poor, represent a major obstacle to personalized medicine.

Over the past decade, the sequencing technology has facilitated a deeper understanding of GBM heterogeneity by “zooming in” more progressive on tumor genomics and transcriptomics. initial efforts to use mass ribonucleic acid (RNA) sequencing, which examined the combined gene expression of tumor specimens as a whole. While innovative at the time, this bulk RNAseq mask important contribution of sub-populations of different tumor’s gene expression as a whole.

This work evolved into mass use RNA sequencing on tumor subsections anatomically and spatially distinct, previously unappreciated complexity exhibited genome of GBM. The revolutionary next step forward has been the emergence of a single cell RNA sequencing (scRNAseq), which examined the gene expression at the single cell level. scRNAseq has enabled us to understand in detail the heterogeneity GBM unprecedented.

We review studies of seminal in the development of our understanding of GBM heterogeneity, with a focus on scRNAseq and insight that have been provided in the understanding of GBM tumor mass, peritumoral space, and TME. We highlight the preclinical and clinical implications of this work and consider the potential impact of neuro-oncology, and to improve patient outcomes through personalized medicine.

[Knockdown of Fez family of zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) inhibits invasion and migration of esophageal squamous cell carcinoma cells by blocking the JAK2 / STAT3 pathway]

Objective To investigate the relationship between the long chain of non-coding RNA Fez family of zinc protein finger 1 antisense ribonucleic acid 1 (FEZF1-AS1) and invasion, migration and Janus kinase 2 / signal transducer and activator of transcription 3 (JAK2 / STAT3) signaling pathway in carcinoma squamous cell (ESCC) cells of the esophagus. Methods The expression ‘of FEZF1-AS1 in cancer tissues and cell lines ESCC ESCC was detected by real-time quantitative PCR.

After knocking out FEZF1-AS1 of KYSE150 and KYSE510 cells with expression vectors lentiviral built, changes in cell cycle were detected by flow cytometry, the ability of cell proliferation by colony test formations, the ability of cell invasion by TranswellTM invasion assay, the ability of cell migration by TranswellTM migration and early test, and the level of phosphorylated proteins JAK2 and STAT3 (p-STAT3) by Western blotting.

Purified Influenza A Neuraminidase (H1N1-NA) protein control for western blot
H1N1NA11-C	Alpha Diagnostics	100 ul	EUR 343.2

NATtrol Influenza Negative Control (6 X 0.5 mL)
NATCXVA9-6MC	Zeptometrix	6 X 0.5 mL	EUR 220

NATtrol Influenza/RSV Negative Control (6 x 0.5mL)
NATCXVA9-6C	Zeptometrix	6 x 0.5mL	EUR 220

NATtrol Influenza/RSV Positive Control (6 x 0.25mL)
NATFLURSV-6L	Zeptometrix	6 x 0.25mL	EUR 272

NATtrol Influenza/RSV Positive Control (6 x 0.5 mL)
NATFLURSV-6C	Zeptometrix	6 x 0.5 mL	EUR 221

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)
NATCXVA9-6C-IVD	Zeptometrix	6 X 0.5 mL	EUR 240

NATtrol Influenza/RSV Negative Control (6 X 0.5 mL)
MDZ045	Zeptometrix	6 X 0.5 mL	EUR 272.16
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza A H1N1, External Run Control, Low (6 X 1 mL)
NATFLUAH1N1-ERCL	Zeptometrix	6 X 1 mL	EUR 360

Influenza Rapid Test Control Pack (3 X 1.5mL)
KZMC033	Zeptometrix	3 X 1.5mL	EUR 59

NATtrol Influenza A H1N1, External Run Control, Medium (6 X 1 mL)
NATFLUAH1N1-ERCM	Zeptometrix	6 X 1 mL	EUR 438

NATtrol Influenza B, External Run Control, Low (6 X 1 mL)
NATFLUB-ERCL	Zeptometrix	6 X 1 mL	EUR 360

Recombinant Purified Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) protein control for Western
H1N1-01-C	Alpha Diagnostics	100 ul	EUR 343.2

NATtrol Influenza B, External Run Control, Medium (6 X 1 mL)
NATFLUB-ERCM	Zeptometrix	6 X 1 mL	EUR 438

Influenza A virus (H5N1) matrix protein 2 Control/blocking peptide
M2E15-P	Alpha Diagnostics	100 ug	EUR 196.8

Influenza A virus (H1N1) matrix protein 2 Control/blocking peptide
M2E16-P	Alpha Diagnostics	100 ug	EUR 196.8

Influenza A virus (H9N2) matrix protein 2 Control/blocking peptide
M2E17-P	Alpha Diagnostics	100 ug	EUR 196.8

Recombinant Purified Hemagglutinin Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) protein control for Western
H11N2-01-C	Alpha Diagnostics	100 ul	EUR 343.2

Influenza A virus (H5N1) matrix protein 2 hybrid sequence Control/blocking peptide
M2E11-P	Alpha Diagnostics	100 ug	EUR 196.8

Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A/chicken/India/NIV33487/2006) (17-531aa) protein control for Western
H5N11-C	Alpha Diagnostics	100 ul	EUR 343.2

Human anti-H. Influenzae B (Hib-PRP) IgG negative control serum
980-101-HNC	Alpha Diagnostics	1 ml	EUR 196.8

Human anti-H. Influenzae B (Hib-PRP) IgG positive control serum
980-101-HPC	Alpha Diagnostics	1 ml	EUR 270

Human anti-H. Influenzae B protein D (Hib-PDG) IgG negative control serum
980-200-01N	Alpha Diagnostics	1 ml	EUR 196.8

Human anti-H. Influenzae B protein D (Hib-PDG) IgG positive control serum
980-200-02P	Alpha Diagnostics	1 ml	EUR 270

Core Panel Multi-Tumor control slides, set of 5
TS900	Innovex	Set of 5	EUR 145
Description: Core Panel Multi-Tumor control slides, set of 5

Core Panel Multi-Tumor control slides, set of 25
TS900-25	Innovex	Set of 25	EUR 425
Description: Core Panel Multi-Tumor control slides, set of 25

Breast Panel Multi-Tumor control slides, set of 5
TS901	Innovex	Set of 5	EUR 145
Description: Breast Panel Multi-Tumor control slides, set of 5

Breast Panel Multi-Tumor control slides, set of 25
TS901-25	Innovex	Set of 25	EUR 395
Description: Breast Panel Multi-Tumor control slides, set of 25

Goat IgG (-ve control for flow cytometry) (isotype control)
20011-100	Alpha Diagnostics	100 test	EUR 123.6

Recombinant H. Influenzae protein D (Non-typeable, NT-PHiD) controls for western blot
PHID11-C	Alpha Diagnostics	100 ul	EUR 343.2

BSA Control for Age-BSA
2221-BSA	Biovision	each	EUR 210

BSA Control for AGE-BSA
35R-AA007	Fitzgerald	10 mg	EUR 268.8
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Flow Control Fitting for Flypad
FLY1254	Scientific Laboratory Supplies	EACH	EUR 101.84

Control for ER, 4 cases (1.5mm)
ERC081	Pantomics	1	EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for PR, 3 cases (1.5mm)
PRC061	Pantomics	1	EUR 102
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

Flow Control Fitting for Blowgun
FLY1252	Scientific Laboratory Supplies	EACH	EUR 80.95

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-2	Alpha Diagnostics	100 ug	EUR 270

Control for DOG1, 3 cases (1.5mm)
DOG1061	Pantomics	1	EUR 102
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for HER2, 4 cases (1.5mm)
HRC081	Pantomics	1	EUR 102
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

NATtrol Influenza Verification Panel (18 x 0.5mL)
NATFVP(XP)-C	Zeptometrix	18 x 0.5mL	EUR 722

Control for Ckit, 6 samples (1.5mm)
CKIT061	Pantomics	1	EUR 102
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

NATtrol Influenza Verification Panel (7 x 1.0 mL)
NATFVP-JAN	Zeptometrix	7 x 1.0 mL	EUR 569.18
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Influenza/RSV Verification Panel (21 x 0.5mL)
NATFRVP-C	Zeptometrix	21 x 0.5mL	EUR 862

SLLK, Control Peptide for TSP1 Inhibitor
HY-P0301	MedChemExpress	1mg	EUR 159.6

Control/Blocking peptide for Human WNT5a
WNT511-P	Alpha Diagnostics	100 ug	EUR 196.8

Rec. Human CD21 protein control for WB
CD21-C	Alpha Diagnostics	100 ul	EUR 343.2

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-100	ScyTek Laboratories	100 Slides	EUR 847.2

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-25	ScyTek Laboratories	25 Slides	EUR 278.4

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-5	ScyTek Laboratories	5 Slides	EUR 117.6

Thiostatin Rat protein, control for western
TSTN11-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse IgG (-ve control for flow cytometry)
20008-1-100	Alpha Diagnostics	100 tests	EUR 123.6

Rabbit IgG (-ve control for flow cytometry)
20009-1-100	Alpha Diagnostics	100 tests	EUR 123.6

Apolipoprotein A-Il, Human Plasma protein control for WB
APOA21-C	Alpha Diagnostics	100 ul	EUR 343.2

NATtrol Respiratory Panel 2 (RP2) Controls (Ea)
NATRPC2-BIO	Zeptometrix	Ea	EUR 550.46
Description: Please contact Gentaur in order to receive the datasheet of the product.

Control Sections for FD NeuroSilver™ Kit
PCS101	FD Neurotechnologies	each	EUR 69.6

Purified, Human S100 protein control for WB
S1002-C	Alpha Diagnostics	100 ul	EUR 343.2

Control/Blocking peptide for Human WNT-1
WNT111-P	Alpha Diagnostics	100 ug	EUR 548.4

Negative Control Antibody for Mouse Monoclonals
V8785-100UG	NSJ Bioreagents	100ug	EUR 349.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Negative Control Antibody for Mouse Monoclonals
V8785-20UG	NSJ Bioreagents	20ug	EUR 153.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

Negative Control Antibody for Mouse Monoclonals
V8785SAF-100UG	NSJ Bioreagents	100ug	EUR 349.3
Description: To serve as a true negative control for various procedures, this monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.

SLLK, Control Peptide for TSP1 Inhibitor(TFA)
HY-P0301A	MedChemExpress	5mg	EUR 456

Purified, Bovine S100 protein control for WB
S1001-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat MEH Antibody
MEHCON	Detroit R&D	100 uL	EUR 150

Positive Control for anti-GST a-forms: rat A1+A2 (Ya)
GSTCON1	Detroit R&D	100 uL	EUR 150

Positive Control for anti-GST a-forms: rat A3+A4 (Yc)
GSTCON11	Detroit R&D	100 uL	EUR 150

Human Pancreatic Amylase protein control for Western blot
PAMY13-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified Human Serum amyloid A (SAA) Protein control for WB
SAA11-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Apolipoprotein B protein control for WB
APOB21-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat CYP2C Antibody
P2CCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP2D Antibody
P2DCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP2B1 Antibody
P2B1PTCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Rat CYP3A2 Antibody
P3A2PTCON	Detroit R&D	100 uL	EUR 150

Haptoglobin Human Plasma protein control for WB
HGLB18-C	Alpha Diagnostics	100 ul	EUR 343.2

Control/Blocking peptide for Mouse Vimentin (Vim)
VIM11-C	Alpha Diagnostics	100 ug	EUR 196.8

NATtrol Respiratory Panel 2.1 (RP2.1) Controls (12 x 0.3mL)
NATRPC2.1-BIO	Zeptometrix	12 x 0.3mL	EUR 529

Mouse IgG Isotype control (purified) validated for
NG903	Innovex	7 ml	EUR 195
Description: Mouse IgG Isotype control (purified) validated for IHC; Ready-To-Use

Mouse IgG Isotype control (purified) validated for
NG903C	Innovex	1 ml	EUR 275
Description: Mouse IgG Isotype control (purified) validated for IHC; Concentrate; Minimum 1:50 dilution

Mouse IgM Isotype control (purified) validated for
NG906	Innovex	7 ml	EUR 265
Description: Mouse IgM Isotype control (purified) validated for IHC; Ready-To-Use

Mouse IgM Isotype control (purified) validated for
NG906C	Innovex	1 ml	EUR 295
Description: Mouse IgM Isotype control (purified) validated for IHC; Concentrate

Positive Control for Anti-Human CYP3A Antibody
P3ACON	Detroit R&D	100 uL	EUR 150

Eppendorf Centrifuge 5430R Knob Control Refrigerated- IVD Only
E5428000465	Scientific Laboratory Supplies	EACH	EUR 8115.6

Control/Blocking peptide for Ephrin-B2 antibody
NIVE2B-C	Alpha Diagnostics	100 ug	EUR 196.8

Positive Control for Anti-Human CYP1B1 Antibody
PH1B1CON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP2E1 Antibody
P2E1PTCON	Detroit R&D	100 uL	EUR 150

Positive Control for Anti-Human CYP1A2 Antibody
P1A2CON	Detroit R&D	100 uL	EUR 150

Chicken Egg Ovalbumin protein control for Western
OVA11-C	Alpha Diagnostics	100 ul	EUR 343.2

Influenza Rapid Test Verification Panel (20 X 0.5 mL)
KZMC021	Zeptometrix	20 X 0.5 mL	EUR 286

Rat Ceruloplasmin protein control for western blot
CP17-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive control tissue section for each individua
Control-slides-	Innovex	Set of 5	EUR 119
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Positive control tissue section for each individua
Control-Slides-for-each-antibody	Innovex	Set of 25	EUR 355
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Control sections for FD NeuroApop™ Kit (frozen)
PCS102-	FD Neurotechnologies	each	EUR 87.6

Control sections for FD NeuroApop™ Kit (frozen)
PCS102-2	FD Neurotechnologies	each	EUR 80.54
Description: Best quality products

Human Native Retinol binding protein (RBP) control for Western
RBP41-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse Ceruloplasmin protein control for western blot
CP16-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified Flagellin (salmonella) protein control for WB
FLGN17-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Hemopexin purified protein control for Western
HPEX11-C	Alpha Diagnostics	100 ul	EUR 343.2

Hemopexin Mouse, purified protein control for Western
HPEX16-C	Alpha Diagnostics	100 ul	EUR 343.2

Q-VE-OPh (Negative Control for Q-VD-OPh)
GW8990-1	Glentham Life Sciences	1	EUR 228.1

Green Fluorescent Proteins (GFP, A. victoria) protein for WB control
GFP11-C	Alpha Diagnostics	100 ul	EUR 343.2

Lectin binding native protein (positive control for SNA lectin)
LBP16-N-1	Alpha Diagnostics	1 ml	EUR 270

Human Apolipoprotein C-I protein control for WB
APOC11-C	Alpha Diagnostics	100 ul	EUR 343.2

Control sections for FD NeuroApop™ Kit (paraffin)
PCS102-1	FD Neurotechnologies	each	EUR 80.44
Description: Best quality products

Purified Human Survivin protein for WB +ve control
SURV11-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified Cholera Toxin A subunit protein control for Western Blot
CTOX16-C	Alpha Diagnostics	100 ul	EUR 343.2

Lectin binding native protein (negative control for all lectins)
LNP15-N-1	Alpha Diagnostics	1 ml	EUR 270

Human Apolipoprotein C-II protein control for WB
APOC22-C	Alpha Diagnostics	100 ul	EUR 343.2

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK21-C	Alpha Diagnostics	100 ul	EUR 343.2

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK22-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat CHP2B1/2 Antibody
P2B12PTCON	Detroit R&D	100 uL	EUR 150

Bovine Adenosine deaminase protein control for Western
ADA11-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for anti-GST p-forms: rat P
GSTCON3	Detroit R&D	100 uL	EUR 150

Human Apolipoprotein C-III protein control for WB
APOC32-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified human Catalase (CATL) protein control for WB
CATL11-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for Anti-Rat CYP1A1/1A2 Antibody
P1ACON	Detroit R&D	100 uL	EUR 150

Purified bovine Lactoferrin protein control for Western
LTF13-C	Alpha Diagnostics	100 ul	EUR 343.2

Rat neurofascin (Nfasc)-control Control/blocking peptide
AB-23249-CP	Alpha Diagnostics	100ug	EUR 196.8

Chicken Egg white proteins total control for Western
CEGW12-C	Alpha Diagnostics	100 ul	EUR 343.2

Rabbit Beta Actin (muscle) protein control for Western
ACTB15-C	Alpha Diagnostics	100 ul	EUR 388.8

Human plasma Apolipoprotein E protein control for WB
APOE13-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Plasma Apolipoprotein J protein control for WB
APOJ13-C	Alpha Diagnostics	100 ul	EUR 343.2

Chicken Egg yellow proteins total control for Western
CEYP12-C	Alpha Diagnostics	100 ul	EUR 343.2

Chicken Serum Albumin protein control for Western blot
ALBC15-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Salivary Amylase protein positive control for WB
SAMY16-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified, recombinant Human TACE control protein for WB
TACE11-C	Alpha Diagnostics	100 ul	EUR 343.2

pMD18- Control
PVT10563	Lifescience Market	2 ug	EUR 319.2

pMD19- Control
PVT10564	Lifescience Market	2 ug	EUR 319.2

Purified Cholera Toxin protein control for Western Blot
CTOX11-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Prothrombin (Factor II) protein control for Western
PRTN11-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse Prothrombin (Factor II) protein control for Western
PRTN13-C	Alpha Diagnostics	100 ul	EUR 343.2

Mouse Angiopoietin 3 (Ang-3) protein control for WB
ANG31-C	Alpha Diagnostics	100 ul	EUR 343.2

Human Angiopoietin 4 (Ang-4) protein control for WB
ANG41-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified Rat C-Reactive Protein (CRP) control for WB
CRP16-C	Alpha Diagnostics	100 ul	EUR 343.2

Purified Dog C-Reactive Protein (CRP) control for WB
CRP18-C	Alpha Diagnostics	100 ul	EUR 343.2

Influenza A H1 Peptide
abx060615-005mg	Abbexa	0.05 mg	EUR 393.6

Influenza A H1 Peptide
abx060616-005mg	Abbexa	0.05 mg	EUR 393.6

Lectin binding native protein (positive control for ConA, WGA lectins)
LBP15-N-1	Alpha Diagnostics	1 ml	EUR 270

Positive Control for Anti-Human Cytochrome b5 Antibody
Cb5CON	Detroit R&D	100 uL	EUR 150

Recombinant (CHO) human DNAse I Protein control for WB
DNASE12-C	Alpha Diagnostics	100 ul	EUR 343.2

Positive Control for anti-GST m-forms: rat M1 (Yb)
GSTCON2	Detroit R&D	100 uL	EUR 150

Quality Control
abx098966-1vial	Abbexa	1 vial	EUR 360

Influenza A H1 Antibody
abx021964-1mg	Abbexa	1 mg	EUR 1111.2

Influenza A H1 Antibody
abx021965-1mg	Abbexa	1 mg	EUR 1111.2

Results FEZF1-AS1 are highly expressed in cancer tissues ESCC and FEZF1-AS1 expression in cells KYSE510 KYSE150 and higher than that in normal esophageal squamous epithelial cells. Upon knockdown FEZF1-AS1 expression, cell cycle and proliferation of cancer cells ESCC did not change obviously, but cell invasion and migration was significantly inhibited, and the protein levels decreased JAK2 and p-STAT3 protein increases. Conclusion FEZF1-AS1 knockdown blocks JAK2 / STAT3 pathway and inhibits cell invasion and migration of ESCC.