Glioblastoma (GBM) is the most common primary brain malignancy in adults and one of the most aggressive of all cancers in humans. It is very repetitive and treatment-resistant, largely because the infiltrative nature and inter- and intratumoral heterogeneity. This heterogeneity requires different landscape genomics and cell types within and between the tumor and the tumor microenvironment (TME). In the GBM, is the driving resistance heterogeneity of treatment, recurrence, and prognosis is poor, represent a major obstacle to personalized medicine.

Over the past decade, the sequencing technology has facilitated a deeper understanding of GBM heterogeneity by “zooming in” more progressive on tumor genomics and transcriptomics. initial efforts to use mass ribonucleic acid (RNA) sequencing, which examined the combined gene expression of tumor specimens as a whole. While innovative at the time, this bulk RNAseq mask important contribution of sub-populations of different tumor’s gene expression as a whole.

This work evolved into mass use RNA sequencing on tumor subsections anatomically and spatially distinct, previously unappreciated complexity exhibited genome of GBM. The revolutionary next step forward has been the emergence of a single cell RNA sequencing (scRNAseq), which examined the gene expression at the single cell level. scRNAseq has enabled us to understand in detail the heterogeneity GBM unprecedented.

We review studies of seminal in the development of our understanding of GBM heterogeneity, with a focus on scRNAseq and insight that have been provided in the understanding of GBM tumor mass, peritumoral space, and TME. We highlight the preclinical and clinical implications of this work and consider the potential impact of neuro-oncology, and to improve patient outcomes through personalized medicine.

[Knockdown of Fez family of zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) inhibits invasion and migration of esophageal squamous cell carcinoma cells by blocking the JAK2 / STAT3 pathway]

Objective To investigate the relationship between the long chain of non-coding RNA Fez family of zinc protein finger 1 antisense ribonucleic acid 1 (FEZF1-AS1) and invasion, migration and Janus kinase 2 / signal transducer and activator of transcription 3 (JAK2 / STAT3) signaling pathway in carcinoma squamous cell (ESCC) cells of the esophagus. Methods The expression ‘of FEZF1-AS1 in cancer tissues and cell lines ESCC ESCC was detected by real-time quantitative PCR.

After knocking out FEZF1-AS1 of KYSE150 and KYSE510 cells with expression vectors lentiviral built, changes in cell cycle were detected by flow cytometry, the ability of cell proliferation by colony test formations, the ability of cell invasion by TranswellTM invasion assay, the ability of cell migration by TranswellTM migration and early test, and the level of phosphorylated proteins JAK2 and STAT3 (p-STAT3) by Western blotting.

NATtrol Influenza A H1, External Run Control, Medium (6 X 1 mL)
NATFLUAH1-ERCM	Zeptometrix	6 X 1 mL	EUR 401.52
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Influenza A virus HA Control/blocking peptide
AB-23091-P	Alpha Diagnostics	100ug	EUR 164

Influenza A H1 Peptide
abx060615-005mg	Abbexa	0.05 mg	EUR 328
Shipped within 5-10 working days.

Influenza A H1 Peptide
abx060616-005mg	Abbexa	0.05 mg	EUR 328
Shipped within 5-10 working days.

Influenza A H1 Antibody
abx021964-1mg	Abbexa	1 mg	EUR 926
Shipped within 5-10 working days.

Influenza A H1 Antibody
abx021965-1mg	Abbexa	1 mg	EUR 926
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Influenza A H1 Antibody
abx021966-1mg	Abbexa	1 mg	EUR 968
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Influenza A H1 Antibody
abx021967-1mg	Abbexa	1 mg	EUR 968
Shipped within 5-10 working days.

Influenza A H1 Antibody
abx021968-1mg	Abbexa	1 mg	EUR 968
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Influenza A H1 Antibody
abx021969-1mg	Abbexa	1 mg	EUR 968
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Influenza A H1 Antibody
abx021970-1mg	Abbexa	1 mg	EUR 968
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Influenza A H1 Antibody
abx023068-100ug	Abbexa	100 ug	EUR 843
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Core Panel Multi-Tumor control slides
TS900	Innovex	Set of 5	EUR 218

Core Panel Multi-Tumor control slides
TS900-25	Innovex	Set of 25	EUR 485

Breast Panel Multi-Tumor control slides
TS901	Innovex	Set of 5	EUR 218

Breast Panel Multi-Tumor control slides
TS901-25	Innovex	Set of 25	EUR 485

Goat IgG (-ve control for flow cytometry) (isotype control)
20011-100	Alpha Diagnostics	100 test	EUR 103

BSA Control for AGE-BSA
35R-AA007	Fitzgerald	10 mg	EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

BSA Control for Age-BSA
2221-BSA	Biovision		EUR 175

Recombinant Purified Hemagglutinin Influenza A Virus (H11N2; A/duck/Yangzhou/906/2002) protein control for Western
H11N2-01-C	Alpha Diagnostics	100 ul	EUR 286

NATtrol Influenza A/B Positive Control (6 x 0.5mL)
NATFLUAB-6C	Zeptometrix	6 x 0.5mL	EUR 226.8
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Influenza A H1 (Swine) Peptide
abx060603-005mg	Abbexa	0.05 mg	EUR 328
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Influenza A HA H1 antibody
10-7839	Fitzgerald	500 ug	EUR 291
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Influenza A HA H1 antibody
10-7840	Fitzgerald	500 ug	EUR 291
Description: Mouse monoclonal Influenza A haemagglutinin H1 antibody

Apolipoprotein A-Il, Human Plasma protein control for WB
APOA21-C	Alpha Diagnostics	100 ul	EUR 286

Control for HER2, 4 cases (1.5mm)
HRC081	Pantomics	1	EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Control for ER, 4 cases (1.5mm)
ERC081	Pantomics	1	EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

SLLK, Control Peptide for TSP1 Inhibitor
HY-P0301	MedChemExpress	1mg	EUR 133

Control for DOG1, 3 cases (1.5mm)
DOG1061	Pantomics	1	EUR 85
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for Ckit, 6 samples (1.5mm)
CKIT061	Pantomics	1	EUR 85
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

Control for PR, 3 cases (1.5mm)
PRC061	Pantomics	1	EUR 85
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-100	ScyTek Laboratories	100 Slides	EUR 706

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-25	ScyTek Laboratories	25 Slides	EUR 232

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-5	ScyTek Laboratories	5 Slides	EUR 98

Thiostatin Rat protein, control for western
TSTN11-C	Alpha Diagnostics	100 ul	EUR 286

Control/Blocking peptide for Human WNT5a
WNT511-P	Alpha Diagnostics	100 ug	EUR 164

Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA
20021-1	Alpha Diagnostics	100 ug	EUR 225

Elk IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-1	Alpha Diagnostics	100 ug	EUR 225

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-2	Alpha Diagnostics	100 ug	EUR 225

Bison IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20025-1	Alpha Diagnostics	100 ug	EUR 225

Raccoon IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20026-1	Alpha Diagnostics	100 ug	EUR 225

Skunk IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20027-1	Alpha Diagnostics	100 ug	EUR 225

NATtrol Influenza A/B Positive Control (6 X 0.5 mL)
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Influenza A virus (H5N1) matrix protein 2 Control/blocking peptide
M2E15-P	Alpha Diagnostics	100 ug	EUR 164

Influenza A virus (H1N1) matrix protein 2 Control/blocking peptide
M2E16-P	Alpha Diagnostics	100 ug	EUR 164

Influenza A virus (H9N2) matrix protein 2 Control/blocking peptide
M2E17-P	Alpha Diagnostics	100 ug	EUR 164

Green Fluorescent Proteins (GFP, A. victoria) protein for WB control
GFP11-C	Alpha Diagnostics	100 ul	EUR 286

Purified Cholera Toxin A subunit protein control for Western Blot
CTOX16-C	Alpha Diagnostics	100 ul	EUR 286

Meningococcal Group A Oligosaccharides-Diphtheria CRM197 conjugate control for ELISA
MENA15-N-100	Alpha Diagnostics	100 ul	EUR 225

Purified Human Serum amyloid A (SAA) Protein control for WB
SAA11-C	Alpha Diagnostics	100 ul	EUR 286

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abx415739-01mg	Abbexa	0.1 mg	EUR 718
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Influenza A virus H1 PCR kit
PCR-H648-48R	Bioingentech	50T	EUR 524.4
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Influenza A virus H1 PCR kit
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Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange	Favorgen	1 prep	EUR 158

Recombinant Borrelia burgdorferi Osp-A protein (OSP-A) control for Western blot
OSPA13-C	Alpha Diagnostics	100 ul	EUR 286

Goat polyclonal antibody for Influenza A
1301	Virostat	1 ml	EUR 272.94
Description: This is goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1303	Virostat	1 ml	EUR 289.25
Description: This is FITC conjugated goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1304	Virostat	1 ml	EUR 311
Description: This is HRP conjugated goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1307	Virostat	1 ml	EUR 289.25
Description: This is Biotin conjugated goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1311	Virostat	1 ml	EUR 272.94
Description: This is goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1313	Virostat	1 ml	EUR 289.25
Description: This is FITC conjugated goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1314	Virostat	1 ml	EUR 311
Description: This is HRP conjugated goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

Goat polyclonal antibody for Influenza A
1317	Virostat	1 ml	EUR 289.25
Description: This is Biotin conjugated goat polyclonal antibody against Influenza A virions, H3N2 for WB, ELISA.

Goat polyclonal antibody for Influenza A
1321	Virostat	1 ml	EUR 272.94
Description: This is goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

Goat polyclonal antibody for Influenza A
1323	Virostat	1 ml	EUR 289.25
Description: This is FITC conjugated goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

Goat polyclonal antibody for Influenza A
1327	Virostat	1 ml	EUR 289.25
Description: This is Biotin conjugated goat polyclonal antibody against Influenza A matrix protein for WB, ELISA.

Mouse antibody for Influenza A virus
1333	Virostat	100 ug	EUR 359.94
Description: This is Mouse monoclonal FITC conjugated antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus
1343	Virostat	100 ug	EUR 359.94
Description: This is Mouse monoclonal FITC conjugated antibody against Influenza A virus NP for WB, ELISA.

Goat polyclonal antibody for Influenza A
1974	Virostat	1 ml	EUR 321.88
Description: This is HRP conjugated goat polyclonal antibody against Influenza A virions, H1N1 for WB, ELISA.

Human Apolipoprotein B protein control for WB
APOB21-C	Alpha Diagnostics	100 ul	EUR 286

Rec. Human CD21 protein control for WB
CD21-C	Alpha Diagnostics	100 ul	EUR 286

Bovine Adenosine deaminase protein control for Western
ADA11-C	Alpha Diagnostics	100 ul	EUR 286

Haptoglobin Human Plasma protein control for WB
HGLB18-C	Alpha Diagnostics	100 ul	EUR 286

Human Hemopexin purified protein control for Western
HPEX11-C	Alpha Diagnostics	100 ul	EUR 286

Hemopexin Mouse, purified protein control for Western
HPEX16-C	Alpha Diagnostics	100 ul	EUR 286

Purified Flagellin (salmonella) protein control for WB
FLGN17-C	Alpha Diagnostics	100 ul	EUR 286

SLLK, Control Peptide for TSP1 Inhibitor(TFA)
HY-P0301A	MedChemExpress	5mg	EUR 380

Mouse IgG (-ve control for flow cytometry)
20008-1-100	Alpha Diagnostics	100 tests	EUR 103

Rabbit IgG (-ve control for flow cytometry)
20009-1-100	Alpha Diagnostics	100 tests	EUR 103

Mouse Ceruloplasmin protein control for western blot
CP16-C	Alpha Diagnostics	100 ul	EUR 286

Rat Ceruloplasmin protein control for western blot
CP17-C	Alpha Diagnostics	100 ul	EUR 286

Control/Blocking peptide for Ephrin-B2 antibody
NIVE2B-C	Alpha Diagnostics	100 ug	EUR 164

Mouse negative control sera (purified) for IHC
NCM802M	Innovex	7 ml	EUR 199

Positive Control for Anti-Human CYP1A2 Antibody
P1A2CON	Detroit R&D	100 uL	EUR 125

Control Sections for FD NeuroSilver™ Kit
PCS101	FD Neurotechnologies	each	EUR 53.83
Description: Best quality products

Positive Control for Anti-Human CYP1B1 Antibody
PH1B1CON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat MEH Antibody
MEHCON	Detroit R&D	100 uL	EUR 125

Purified bovine Lactoferrin protein control for Western
LTF13-C	Alpha Diagnostics	100 ul	EUR 286

Chicken Egg Ovalbumin protein control for Western
OVA11-C	Alpha Diagnostics	100 ul	EUR 286

Positive Control for Anti-Rat CYP2B1 Antibody
P2B1PTCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP2C Antibody
P2CCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP2D Antibody
P2DCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Human CYP2E1 Antibody
P2E1PTCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP3A2 Antibody
P3A2PTCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Human CYP3A Antibody
P3ACON	Detroit R&D	100 uL	EUR 125

Purified, Bovine S100 protein control for WB
S1001-C	Alpha Diagnostics	100 ul	EUR 286

Purified, Human S100 protein control for WB
S1002-C	Alpha Diagnostics	100 ul	EUR 286

Control/Blocking peptide for Mouse Vimentin (Vim)
VIM11-C	Alpha Diagnostics	100 ug	EUR 164

Control/Blocking peptide for Human WNT-1
WNT111-P	Alpha Diagnostics	100 ug	EUR 457

Rabbit anti Arabidopsis thaliana Actin-1 (ACT1) IgG affinity pure, loading control for WB
PACT11-A	Alpha Diagnostics	100 ug	EUR 457

Positive Control for anti-GST a-forms: rat A1+A2 (Ya)
GSTCON1	Detroit R&D	100 uL	EUR 125

Positive Control for anti-GST a-forms: rat A3+A4 (Yc)
GSTCON11	Detroit R&D	100 uL	EUR 125

Control siRNA Vector (pGB-control)
9500C-20	Biovision		EUR 338

Recombinant purified Rat Toolan's H1 parvovirus (THV) capsid protein VP2 control for Western blot
THVP11-C	Alpha Diagnostics	100 ul	EUR 286

Rabbit Anti-Hemagglutinin Influenza A Virus H1N1 H1 (H1N1) (A/New Caledonia/20/99) IgG
H1N1-01-A	Alpha Diagnostics	100 ul	EUR 482

Recombinant Purified Hemagglutinin Influenza A Virus H5N1 H5 (H5N1) (A/chicken/India/NIV33487/2006) (17-531aa) protein control for Western
H5N11-C	Alpha Diagnostics	100 ul	EUR 286

Influenza A virus H1 RT PCR kit
RTq-H648-100R	Bioingentech	100T	EUR 776.4
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Description: A Real-Time PCR kit for detection of Influenza A virus H1 .

Influenza A virus H1 RT PCR kit
RTq-H648-150R	Bioingentech	150T	EUR 877.9
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Description: A Real-Time PCR kit for detection of Influenza A virus H1 .

Influenza A virus H1 RT PCR kit
RTq-H648-50R	Bioingentech	50T	EUR 631.4
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Description: A Real-Time PCR kit for detection of Influenza A virus H1 .

pSIF1-H1-siLuc-copGFP Positive Transduction Control (plasmid)
LV201PB-1	SBI	10 ug	EUR 625
Category: Lentiviral Technology

pSIH1-H1-siLuc-copGFP Packaged Positive Transduction Control
LV601B-1	SBI	>1 x 10^6 IFUs	EUR 699
Category: Lentiviral Technology

pSIH1-H1-siLuc-copGFP positive transduction control, plasmid
LV601PB-1	SBI	10 ug	EUR 625
Category: Lentiviral Technology

NATtrol Influenza/RSV Negative Control (6 x 0.5mL)
NATCXVA9-6C	Zeptometrix	6 x 0.5mL	EUR 226.8
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NATtrol Influenza Negative Control (6 X 0.5 mL)
NATCXVA9-6MC	Zeptometrix	6 X 0.5 mL	EUR 232
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NATtrol Influenza/RSV Positive Control (6 x 0.25mL)
NATFLURSV-6L	Zeptometrix	6 x 0.25mL	EUR 268.4
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Influenza Rapid Test Control Pack (3 X 1.5mL)
KZMC033	Zeptometrix	3 X 1.5mL	EUR 97.84
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Mouse antibody for Influenza A virus NP
1331	Virostat	100 ug	EUR 321.88
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1341	Virostat	100 ug	EUR 321.88
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1361	Virostat	100 ug	EUR 321.88
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1371	Virostat	100 ug	EUR 321.88
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus NP
1381	Virostat	100 ug	EUR 321.88
Description: This is purified Mouse monoclonal antibody against Influenza A virus NP for WB, ELISA.

Mouse antibody for Influenza A virus H5
1385	Virostat	100 ug	EUR 321.88
Description: This is purified Mouse monoclonal antibody against Influenza A virus H5 for WB, ELISA.

NATtrol Influenza A H1N1, External Run Control, Low (6 X 1 mL)
NATFLUAH1N1-ERCL	Zeptometrix	6 X 1 mL	EUR 339.12
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NATtrol Influenza A H1N1, External Run Control, Medium (6 X 1 mL)
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Influenza A virus (H5N1) matrix protein 2 hybrid sequence Control/blocking peptide
M2E11-P	Alpha Diagnostics	100 ug	EUR 164

Rabbit anti Arabidopsis thaliana Tubulin alpha-1 chain (TUBA1) IgG affinity pure, loading control for WB
TUBA111-A	Alpha Diagnostics	100 ug	EUR 457

Rabbit anti Arabidopsis thaliana Tubulin beta-2 chain (TUBB2) IgG affinity pure, loading control for WB
TUBB211-A	Alpha Diagnostics	100 ug	EUR 457

Chicken Serum Albumin protein control for Western blot
ALBC15-C	Alpha Diagnostics	100 ul	EUR 286

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK21-C	Alpha Diagnostics	100 ul	EUR 286

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK22-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein C-I protein control for WB
APOC11-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein C-II protein control for WB
APOC22-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein C-III protein control for WB
APOC32-C	Alpha Diagnostics	100 ul	EUR 286

Human plasma Apolipoprotein E protein control for WB
APOE13-C	Alpha Diagnostics	100 ul	EUR 286

Human Plasma Apolipoprotein J protein control for WB
APOJ13-C	Alpha Diagnostics	100 ul	EUR 286

Purified human Catalase (CATL) protein control for WB
CATL11-C	Alpha Diagnostics	100 ul	EUR 286

Positive Control for Anti-Human Cytochrome b5 Antibody
Cb5CON	Detroit R&D	100 uL	EUR 125

Chicken Egg Conalbumin (ovotransferrin) protein control for Western
CECA15-C	Alpha Diagnostics	100 ul	EUR 286

Chicken Egg white proteins total control for Western
CEGW12-C	Alpha Diagnostics	100 ul	EUR 286

Results FEZF1-AS1 are highly expressed in cancer tissues ESCC and FEZF1-AS1 expression in cells KYSE510 KYSE150 and higher than that in normal esophageal squamous epithelial cells. Upon knockdown FEZF1-AS1 expression, cell cycle and proliferation of cancer cells ESCC did not change obviously, but cell invasion and migration was significantly inhibited, and the protein levels decreased JAK2 and p-STAT3 protein increases. Conclusion FEZF1-AS1 knockdown blocks JAK2 / STAT3 pathway and inhibits cell invasion and migration of ESCC.